Müller Anja, Seinige Diana, Grabowski Nils T, Ahlfeld Birte, Yue Min, Kehrenberg Corinna
Institute for Veterinary Food Science, Justus Liebig University Giessen, Frankfurter Str. 92, 35392 Giessen, Germany.
Lower Saxony State Office for Consumer Protection and Food Safety, 26203 Wardenburg, Germany.
Foods. 2021 Oct 22;10(11):2552. doi: 10.3390/foods10112552.
Insects as novel foods are gaining popularity in Europe. Regulation (EU) 2015/2283 laid the framework for the application process to market food insects in member states, but potential hazards are still being evaluated. The aim of this study was to investigate samples of edible insect species for the presence of antimicrobial-resistant and Shiga toxin-producing (STEC). Twenty-one isolates, recovered from samples of five different edible insect species, were subjected to antimicrobial susceptibility testing, PCR-based phylotyping, and macrorestriction analysis. The presence of genes associated with antimicrobial resistance or virulence, including , and , was investigated by PCR. All isolates were subjected to genome sequencing, multilocus sequence typing, and serotype prediction. The isolates belonged either to phylogenetic group A, comprising mostly commensal , or group B1. One O178:H7 isolate, recovered from a sample, was identified as a STEC. A single isolate was resistant to tetracyclines and carried the (B) gene. Overall, this study shows that STEC can be present in edible insects, representing a potential health hazard. In contrast, the low resistance rate among the isolates indicates a low risk for the transmission of antimicrobial-resistant to consumers.
昆虫作为新型食物在欧洲越来越受欢迎。欧盟法规（EU）2015/2283为成员国食品昆虫上市的申请流程奠定了框架，但潜在危害仍在评估中。本研究的目的是调查食用昆虫物种样本中是否存在耐抗菌药物和产志贺毒素的菌株（STEC）。从五种不同食用昆虫物种的样本中分离出21株菌株，进行抗菌药物敏感性测试、基于PCR的系统发育分型和宏观限制性分析。通过PCR研究与抗菌药物耐药性或毒力相关的基因，包括、和的存在情况。所有分离株均进行全基因组测序、多位点序列分型和血清型预测。这些分离株要么属于主要由共生组成的系统发育组A，要么属于B1组。从一个样本中分离出的一株O178:H7菌株被鉴定为STEC。有一株分离株对四环素耐药并携带（B）基因。总体而言，本研究表明食用昆虫中可能存在STEC，这代表着一种潜在的健康危害。相比之下，分离株中较低的耐药率表明耐抗菌药物向消费者传播的风险较低。
