Haas Alexis J, Le Page Yann, Zhadobov Maxim, Sauleau Ronan, Dréan Yves Le, Saligaut Christian
Institut national de la santé et de la recherche médicale (Inserm), Institute for Research on Environmental and Occupational Health (IRSET), Inserm UMR1085, 9, avenue du Prof. Léon Bernard, 35 000 Rennes, France.
University of Rennes 1, F-35000 Rennes, France.
J Radiat Res. 2017 Jul 1;58(4):439-445. doi: 10.1093/jrr/rrx004.
Several forthcoming wireless telecommunication systems will use electromagnetic frequencies at millimeter waves (MMWs), and technologies developed around the 60-GHz band will soon know a widespread distribution. Free nerve endings within the skin have been suggested to be the targets of MMW therapy which has been used in the former Soviet Union. So far, no studies have assessed the impact of MMW exposure on neuronal metabolism. Here, we investigated the effects of a 24-h MMW exposure at 60.4 GHz, with an incident power density (IPD) of 5 mW/cm², on the dopaminergic turnover of NGF-treated PC12 cells. After MMW exposure, both intracellular and extracellular contents of dopamine (DA) and 3,4-dihydroxyphenylacetic acid (DOPAC) were studied using high performance liquid chromatography. Impact of exposure on the dopamine transporter (DAT) expression was also assessed by immunocytochemistry. We analyzed the dopamine turnover by assessing the ratio of DOPAC to DA, and measuring DOPAC accumulation in the medium. Neither dopamine turnover nor DAT protein expression level were impacted by MMW exposure. However, extracellular accumulation of DOPAC was found to be slightly increased, but not significantly. This result was related to the thermal effect, and overall, no evidence of non-thermal effects of MMW exposure were observed on dopamine metabolism.
几种即将出现的无线通信系统将使用毫米波（MMW）频段的电磁频率，围绕60吉赫兹频段开发的技术很快将得到广泛应用。皮肤内的游离神经末梢被认为是前苏联曾使用的MMW疗法的作用靶点。到目前为止，尚无研究评估MMW暴露对神经元代谢的影响。在此，我们研究了在60.4吉赫兹、入射功率密度（IPD）为5毫瓦/平方厘米的条件下，24小时MMW暴露对经神经生长因子（NGF）处理的PC12细胞多巴胺能周转的影响。MMW暴露后，使用高效液相色谱法研究了多巴胺（DA）和3,4-二羟基苯乙酸（DOPAC）的细胞内和细胞外含量。还通过免疫细胞化学评估了暴露对多巴胺转运体（DAT）表达的影响。我们通过评估DOPAC与DA的比值并测量培养基中DOPAC的积累来分析多巴胺周转。MMW暴露既未影响多巴胺周转，也未影响DAT蛋白表达水平。然而，发现DOPAC的细胞外积累略有增加，但不显著。该结果与热效应有关，总体而言，未观察到MMW暴露对多巴胺代谢有非热效应的证据。
