Mishra Neelam, Sun Li, Zhu Xunlu, Smith Jennifer, Prakash Srivastava Anurag, Yang Xiaojie, Pehlivan Necla, Esmaeili Nardana, Luo Hong, Shen Guoxin, Jones Don, Auld Dick, Burke John, Payton Paxton, Zhang Hong
Department of Biological Sciences, Texas Tech University, Lubbock, TX, USA.
Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
Plant Cell Physiol. 2017 Apr 1;58(4):735-746. doi: 10.1093/pcp/pcx032.
The Arabidopsis SUMO E3 ligase gene AtSIZ1 plays important roles in plant response to abiotic stresses as loss of function in AtSIZ1 leads to increased sensitivity to drought, heat and salt stresses. Overexpression of the AtSIZ1 rice homolog, OsSIZ1, leads to increased heat and drought tolerance in bentgrass, suggesting that the function of the E3 ligase SIZ1 is highly conserved in plants and it plays a critical role in abiotic stress responses. To test the possibility that the SUMO E3 ligase could be used to engineer drought- and heat-tolerant crops, the rice gene OsSIZ1 was overexpressed in cotton. We report here that overexpression of OsSIZ1 in cotton results in higher net photosynthesis and better growth than wild-type cotton under drought and thermal stresses in growth chamber and greenhouse conditions. Additionally, this tolerance to abiotic stresses was correlated with higher fiber yield in both controlled-environment and field trials carried out under reduced irrigation and rainfed conditions. These results suggest that OsSIZ1 is a viable candidate gene to improve crop yields under water-limited and rainfed agricultural production systems.
拟南芥SUMO E3连接酶基因AtSIZ1在植物对非生物胁迫的响应中发挥重要作用，因为AtSIZ1功能缺失会导致对干旱、高温和盐胁迫的敏感性增加。AtSIZ1水稻同源基因OsSIZ1的过表达导致翦股颖的耐热性和耐旱性增强，这表明E3连接酶SIZ1的功能在植物中高度保守，并且在非生物胁迫响应中起关键作用。为了测试SUMO E3连接酶可用于培育耐旱和耐热作物的可能性，将水稻基因OsSIZ1在棉花中过表达。我们在此报告，在生长室和温室条件下的干旱和热胁迫下，棉花中OsSIZ1的过表达导致其净光合作用更高，生长状况优于野生型棉花。此外，在减少灌溉和雨养条件下进行的控制环境试验和田间试验中，这种对非生物胁迫的耐受性与更高的纤维产量相关。这些结果表明，OsSIZ1是在水分有限和雨养农业生产系统下提高作物产量的一个可行候选基因。
