Weber Michael, Schaer Jonas, Walther Grit, Kaerger Kerstin, Steinmann Jörg, Rath Peter-Michael, Spiess Birgit, Buchheidt Dieter, Hamprecht Axel, Kurzai Oliver
German National Reference Center for Invasive Fungal Infections - NRZMyk, Septomics Research Centre, Leibniz-Institute for Natural Product Research and Infection Biology - Hans-Knoell-Institute, 07745 Jena, Germany.
Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.
Med Mycol. 2018 Jan 1;56(1):117-120. doi: 10.1093/mmy/myx015.
Therapy of invasive aspergillosis is becoming more difficult due to the emergence of azole resistance in Aspergillus fumigatus. A majority of resistant strains carries mutations in the CYP51A gene. Due to a lack of sensitivity of culture-based methods, molecular detection of A. fumigatus has become an important diagnostic tool. We set up the database FunResDB (www.nrz-myk.de/funresdb) to gather all available information about CYP51A-dependent azole resistance from published literature. In summary, the screening resulted in 79 CYP51A variants, which are linked to 59 nonsynonymous mutations. A tailor-made online sequence analysis tool allows for genotypic susceptibility testing of A. fumigatus.
由于烟曲霉中出现唑类耐药性，侵袭性曲霉病的治疗变得愈发困难。大多数耐药菌株的CYP51A基因发生了突变。由于基于培养的方法缺乏敏感性，烟曲霉的分子检测已成为一种重要的诊断工具。我们建立了FunResDB数据库（www.nrz-myk.de/funresdb），以收集已发表文献中所有关于CYP51A依赖性唑类耐药性的可用信息。总之，筛选出了79个CYP51A变体，它们与59个非同义突变相关。一个定制的在线序列分析工具可用于烟曲霉的基因型药敏试验。
