Yang Celina, Uertz Jamie, Chithrani Devika B
Department of Physics, Ryerson University, Toronto, ON M5B 2K3, Canada.
CytoViva Inc., 570 Devall Drive, Auburn, AL 36832, USA.
Nanomaterials (Basel). 2016 Mar 10;6(3):48. doi: 10.3390/nano6030048.
Nanoparticles (NPs) can be used to overcome the side effects of poor distribution of anticancer drugs. Among other NPs, colloidal gold nanoparticles (GNPs) offer the possibility of transporting major quantities of drugs due to their large surface-to-volume ratio. This is while confining these anticancer drugs as closely as possible to their biological targets through passive and active targeting, thus ensuring limited harmful systemic distribution. In this study, we chose to use bleomycin (BLM) as the anticancer drug due to its limited therapeutic efficiency (harmful side effects). BLM was conjugated onto GNPs through a thiol bond. The effectiveness of the chemotherapeutic drug, BLM, is observed by visualizing DNA double strand breaks and by calculating the survival fraction. The action of the drug (where the drug takes effect) is known to be in the nucleus, and our experiments have shown that some of the GNPs carrying BLM were present in the nucleus. The use of GNPs to deliver BLM increased the delivery and therapeutic efficacy of the drug. Having a better control over delivery of anticancer drugs using GNPs will establish a more successful NP-based platform for a combined therapeutic approach. This is due to the fact that GNPs can also be used as radiation dose enhancers in cancer research.
纳米颗粒（NPs）可用于克服抗癌药物分布不佳的副作用。在其他纳米颗粒中，胶体金纳米颗粒（GNPs）因其较大的表面体积比而提供了运输大量药物的可能性。与此同时，通过被动和主动靶向将这些抗癌药物尽可能紧密地定位到其生物靶点，从而确保有害的全身分布有限。在本研究中，由于博来霉素（BLM）治疗效率有限（存在有害副作用），我们选择将其用作抗癌药物。通过硫醇键将BLM偶联到GNPs上。通过观察DNA双链断裂和计算存活分数来观察化疗药物BLM的有效性。已知该药物的作用部位（药物起效的位置）在细胞核，我们的实验表明，一些携带BLM的GNPs存在于细胞核中。使用GNPs递送BLM提高了药物的递送和治疗效果。更好地控制使用GNPs递送抗癌药物将为联合治疗方法建立一个更成功的基于纳米颗粒的平台。这是因为GNPs在癌症研究中也可用作辐射剂量增强剂。
