Wagner George, Korenkov Victor, Judy Jonathan D, Bertsch Paul M
Department of Plant and Soil Sciences, University of Kentucky, Lexington 40546, KY, USA.
CSIRO Land and Water, Urrbrae 5064, Australia.
Nanomaterials (Basel). 2016 Mar 16;6(3):50. doi: 10.3390/nano6030050.
Manufactured nanoparticles (NPs) are increasingly being used for commercial purposes and certain NP types have been shown to have broad spectrum antibacterial activity. In contrast, their activities against fungi and fungi-like oomycetes are less studied. Here, we examined the potential of two types of commercially available Zn NPs (Zn NPs and ZnO NPs) to inhibit spore germination and infectivity on tobacco leaves resulting from exposure to the fungi-like oomycete pathogen (). Both types of NPs, as well as ZnCl₂ and bulk ZnO control treatments, inhibited spore germination compared to a blank control. ZnO ENMs were shown to be a much more powerful suppressor of spore germination and infectivity than bulk ZnO. ZnO and Zn NPs significantly inhibited leaf infection at 8 and 10 mg·L, respectively. Both types of NPs were found to provide substantially higher concentration dependent inhibition of spore germination and infectivity than could be readily explained by the presence of dissolved Zn. These results suggest that both NP types have potential for use as economic, low-dose, potentially non-persistent anti-microbial agents against the oomycete .
人造纳米颗粒（NPs）正越来越多地用于商业目的，并且某些类型的纳米颗粒已被证明具有广谱抗菌活性。相比之下，它们对真菌和类真菌卵菌的活性研究较少。在此，我们研究了两种市售锌纳米颗粒（Zn NPs和ZnO NPs）抑制类真菌卵菌病原体暴露后在烟草叶片上的孢子萌发和侵染性的潜力。与空白对照相比，两种类型的纳米颗粒以及ZnCl₂和块状ZnO对照处理均抑制了孢子萌发。已表明ZnO工程纳米材料比块状ZnO对孢子萌发和侵染性的抑制作用要强得多。ZnO和Zn NPs分别在8和10 mg·L时显著抑制叶片感染。发现两种类型的纳米颗粒对孢子萌发和侵染性的抑制作用在浓度依赖性上比溶解态锌的存在所能轻易解释的要强得多。这些结果表明，两种类型的纳米颗粒都有潜力作为经济、低剂量、可能非持久性的抗卵菌抗菌剂。
