Hassan Basri Hasbullah, Talib Rosnita A, Sukor Rashidah, Othman Siti Hajar, Ariffin Hidayah
Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, Serdang 43400, Malaysia.
Halal Products Research Institute, Universiti Putra Malaysia, Serdang 43400, Malaysia.
Nanomaterials (Basel). 2020 May 30;10(6):1061. doi: 10.3390/nano10061061.
This research investigated the effect of synthesis temperature on the size and shape of zinc oxide (ZnO) nanoparticles (NPs) synthesized using pineapple peel waste and antibacterial activity of ZnO NPs in starch films. Zinc oxide NPs synthesized at different temperatures were characterized by Fourier transform infrared spectroscopy, X-ray diffraction analysis, field-emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, and transmission electron microscopy. Micrographs of ZnO NPs synthesized at 28 and 60 °C showed that synthesis temperature affected the sizes and shapes of ZnO NPs. The non-heated (28 °C) condition resulted in NPs with diameters in the range of 8-45 nm with a mixture of spherical and rod shapes, whereas the heated (60 °C) condition led to NPs with diameters in the range of 73-123 nm with flower rod shapes. The ZnO-starch nanocomposite films incorporated with 1, 3, and 5 wt.% ZnO NPs were prepared via a film casting method. The antibacterial activity of the films against Gram-positive and Gram-negative bacteria was investigated using the disc diffusion method. The results showed an increase in the inhibition zone for Gram-positive bacteria, particularly , when the concentration of ZnO NPs incorporated in the film was increased from 1 to 5 wt.%.
本研究考察了合成温度对以菠萝皮废料合成的氧化锌（ZnO）纳米颗粒（NPs）的尺寸和形状的影响，以及ZnO NPs在淀粉膜中的抗菌活性。通过傅里叶变换红外光谱、X射线衍射分析、场发射扫描电子显微镜、能量色散X射线光谱和透射电子显微镜对在不同温度下合成的ZnO NPs进行了表征。在28℃和60℃合成的ZnO NPs的显微照片表明，合成温度影响了ZnO NPs的尺寸和形状。未加热（28℃）条件下得到的NPs直径在8 - 45nm范围内，形状为球形和棒状的混合物，而加热（60℃）条件下得到的NPs直径在73 - 123nm范围内，形状为花杆状。通过流延法制备了含有1 wt.%、3 wt.%和5 wt.% ZnO NPs的ZnO-淀粉纳米复合膜。采用纸片扩散法研究了该膜对革兰氏阳性菌和革兰氏阴性菌的抗菌活性。结果表明，当膜中ZnO NPs的浓度从1 wt.%增加到5 wt.%时，革兰氏阳性菌的抑菌圈增大，尤其是[此处原文缺失具体细菌名称]。
