Sannat Chandrahas, Patyal Anil, Rawat Nidhi, Ghosh R C, Jolhe D K, Shende R K, Hirpurkar S D, Shakya Sanjay
Department of Veterinary Microbiology, College of Veterinary Science & Animal Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora, Durg - 491 001, Chhattisgarh, India.
Department of Veterinary Public Health, College of Veterinary Science & Animal Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora, Durg - 491 001, Chhattisgarh, India.
Vet World. 2017 Feb;10(2):144-148. doi: 10.14202/vetworld.2017.144-148. Epub 2017 Feb 5.
The present investigation was conducted to isolate and characterize Gallinarum from an outbreak of fowl typhoid in layer birds.
Clinically ill and dead layer birds from an outbreak were investigated. History, clinical signs, and postmortem lesions were suggestive of fowl typhoid. Postmortem samples including heart blood, intestinal contents, pieces of ovary, and liver were collected and processed immediately for bacterial culture, serotyping and antibiotic sensitivity tests. Isolates were further screened for the presence of extended spectrum beta lactamase (ESBL) () gene by polymerase chain reaction.
On the basis of cultural, staining and biochemical characteristics; three bacterial isolates were confirmed as S. Gallinarum. On serotyping, somatic antigen O: 9 and 12 with nonflagellated antigen were detected in all three isolates. Isolates were intermediate sensitive to amoxycillin, amoxyclav, gentamicin and ciprofloxacin and resistant to most of the antibiotics including chloramphenicol, ampicillin, ceftazidime, cefexime, cefepime, azithromycin, nalidixin, tetracycline, oxytetracycline, and streptomycin. Two isolates were found to harbor ESBL () gene.
Beta lactamase producer S. Gallinarum was confirmed as cause of increased mortality in layer birds during present investigation. Existence of multi drug resistant poses serious threat to poultry industry in Chhattisgarh.
进行本次调查以从蛋鸡爆发的禽伤寒疫情中分离并鉴定鸡沙门氏菌。
对疫情中临床患病和死亡的蛋鸡进行调查。病史、临床症状和死后病变提示为禽伤寒。采集死后样本，包括心血、肠内容物、卵巢组织块和肝脏，立即进行细菌培养、血清分型和抗生素敏感性试验。通过聚合酶链反应进一步筛选分离株中是否存在超广谱β-内酰胺酶（ESBL）基因。
根据培养、染色和生化特性，确认三株细菌分离株为鸡沙门氏菌。血清分型显示，所有三株分离株均检测到体细胞抗原O:9和12以及无鞭毛抗原。分离株对阿莫西林、阿莫西林克拉维酸、庆大霉素和环丙沙星中度敏感，对包括氯霉素、氨苄西林、头孢他啶、头孢克肟、头孢吡肟、阿奇霉素、萘啶酸、四环素、土霉素和链霉素在内的大多数抗生素耐药。发现两株分离株携带ESBL基因。
在本次调查中，产β-内酰胺酶的鸡沙门氏菌被确认为蛋鸡死亡率增加的原因。多重耐药菌的存在对恰蒂斯加尔邦的家禽业构成严重威胁。
