Hernández Villamizar Santiago, Bonilla Juliana Arias, Jaramillo Álvaro Hugo, Piñeros Ricardo, Ripoll Antonio, Fonseca Laura, Riveros Karen, Vives Martha J, Barato Paola, Clavijo Viviana
Ciencia y Tecnología de Fagos Sciphage SAS, Mosquera, Colombia.
Departamento de Ciencias Biológicas, Universidad de los Andes, Bogotá, Colombia.
Phage (New Rochelle). 2024 Sep 16;5(3):143-152. doi: 10.1089/phage.2024.0010. eCollection 2024 Sep.
The avian pathogen Gallinarum causes avian typhosis in laying hens, leading to high mortality rates among adult birds, which poses a significant problem in the poultry industry. Various products, such as vaccines, antibiotics, probiotics, and disinfectants, are commonly used to prevent and control the disease on farms. An alternative to these products is the use of bacteriophages, which may effectively prevent the colonization of Gallinarum.
This study evaluated the safety of SalmoFree, a bacteriophage cocktail, administered to 276 laying hens from the first week of age until the 28th week. The hens were divided into two groups: a control group (138 birds) and a treatment group (138 birds). Over the 28-week period, eight doses of SalmoFree (∼10 UFP per bird) were administered via drinking water in a controlled environment.
The results indicate that the consumption of SalmoFree has no adverse effects on bird health or zootechnical parameters. Additionally, there is a trend toward improving weight homogeneity (up to 19%), feed conversion (up to 68%), and egg weight (up to 2.7%). The detection of phages by PCR in cloacal swabs suggests that they persist in birds for 2 to 8 weeks post-ingestion. Furthermore, phages were detected in organs and eggshells, indicating that they provide protection beyond the gut.
The study demonstrates that SalmoFree is safe for use in laying hens and may offer additional benefits, such as improved zootechnical parameters and extended protection against S. Gallinarum through the persistence of bacteriophages in the birds.
禽病原菌鸡伤寒沙门氏菌可导致蛋鸡发生禽伤寒，致使成年鸡死亡率高，这在家禽业中是一个重大问题。农场中常用各种产品，如疫苗、抗生素、益生菌和消毒剂来预防和控制该病。这些产品的一种替代方法是使用噬菌体，其可能有效防止鸡伤寒沙门氏菌的定植。
本研究评估了一种噬菌体鸡尾酒制剂SalmoFree对276只蛋鸡从1周龄至28周龄的安全性。将母鸡分为两组：对照组（138只鸡）和治疗组（138只鸡）。在28周的时间里，在可控环境中通过饮水给鸡施用八剂SalmoFree（每只鸡约10个噬菌斑形成单位）。
结果表明，食用SalmoFree对鸡的健康或畜牧学参数没有不良影响。此外，有改善体重均匀度（高达19%）、饲料转化率（高达68%）和蛋重（高达2.7%）的趋势。通过PCR在泄殖腔拭子中检测到噬菌体，表明它们在摄入后在鸡体内持续存在2至8周。此外，在器官和蛋壳中检测到噬菌体，表明它们提供了超出肠道的保护。
该研究表明SalmoFree用于蛋鸡是安全的，并且可能提供额外的益处，如改善畜牧学参数以及通过噬菌体在鸡体内的持续存在对鸡伤寒沙门氏菌提供延长的保护。
