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多色生物成像用生物合成锌纳米粒子及其在肿瘤检测中的应用。

Multicolor bioimaging with biosynthetic zinc nanoparticles and their application in tumor detection.

机构信息

Wuxi School of Medicine, Jiangnan University, 214122, China.

Department of Cell Biology, School of Basic Medical Sciences, Nanjing Medical University, 210029, China.

出版信息

Sci Rep. 2017 Mar 27;7:45313. doi: 10.1038/srep45313.

DOI:10.1038/srep45313
PMID:28345657
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5366897/
Abstract

Because they generate excellent images, nanoparticles (NPs), especially biosynthesized NPs, provide a new solution for tumor imaging. In this research, we unveil a novel type of biosynthesized NPs featuring multicolor fluorescence. These NPs exhibit little cytotoxicity to cells. The explored NPs, designated Zn-ZFP-GST NPs (Zinc NPs in abbreviation), are generated from leukemia cells treated with a Zn solution, while zinc-finger protein and glutathione S-transferase (GST) were also identified in the Zinc NPs. Under near-UV illumination, the Zinc NPs simultaneously emit green, yellow, and red fluorescence. In addition, the intensity of the fluorescence increases with the existence of sulfides. Besides, the NPs are encapsulated by microvesicles (MVs) shed from the plasma membrane. As observed in whole-body research of nude mice, the NP-MVs migrate via blood circulation and are distinguished by their fluorescent signals. Furthermore, the folic acid (FA) &AVR2 (human VEGF antibody)-coated NP-MVs are exploited to target the tumor location, and the feasibility of this approach has been confirmed empirically. The Zinc NPs shed light on an alternative solution to tumor detection.

摘要

由于纳米粒子（NPs）能生成极好的图像，特别是生物合成的 NPs，为肿瘤成像提供了新的解决方案。在这项研究中，我们揭示了一种新型的具有多色荧光的生物合成 NPs。这些 NPs 对细胞几乎没有细胞毒性。所研究的 NPs 被命名为 Zn-ZFP-GST NPs（简称 Zinc NPs），是从用 Zn 溶液处理的白血病细胞中产生的，而锌指蛋白和谷胱甘肽 S-转移酶（GST）也在 Zinc NPs 中被鉴定出来。在近紫外光照射下，Zinc NPs 同时发出绿色、黄色和红色荧光。此外，荧光强度随硫化物的存在而增加。此外，这些 NPs 被细胞膜释放的微泡（MVs）包裹。在裸鼠的全身研究中观察到，NP-MVs 通过血液循环迁移，并通过其荧光信号来区分。此外，叶酸（FA）和 AVR2（人 VEGF 抗体）涂层的 NP-MVs 被用于靶向肿瘤部位，并且已经通过经验证实了这种方法的可行性。Zinc NPs 为肿瘤检测提供了一种替代解决方案。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed7/5366897/1394c9658a70/srep45313-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed7/5366897/1e614f08a03d/srep45313-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed7/5366897/d765f1ea71a6/srep45313-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed7/5366897/4ce372a57caf/srep45313-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed7/5366897/9f7a43d8664d/srep45313-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed7/5366897/ca50d7f3a5fc/srep45313-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed7/5366897/9bc9ff4cbf80/srep45313-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed7/5366897/1394c9658a70/srep45313-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed7/5366897/1e614f08a03d/srep45313-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed7/5366897/d765f1ea71a6/srep45313-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed7/5366897/4ce372a57caf/srep45313-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed7/5366897/9f7a43d8664d/srep45313-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed7/5366897/ca50d7f3a5fc/srep45313-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed7/5366897/9bc9ff4cbf80/srep45313-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aed7/5366897/1394c9658a70/srep45313-f7.jpg

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