Friedrichs Anna, Busch Julia Anke, van der Woerd Hendrik Jan, Zielinski Oliver
Institute for Chemistry and Biology of the Marine Environment, Carl von Ossietzky University Oldenburg, Schleusenstrasse 1, 26382 Wilhelmshaven, Germany.
Life Sciences and Chemistry, Jacobs University, Campus Ring 1, 28759 Bremen, Germany.
Sensors (Basel). 2017 Mar 25;17(4):678. doi: 10.3390/s17040678.
In order to increase the monitoring capabilities of inland and coastal waters, there is a need for new, affordable, sensitive and mobile instruments that could be operated semi-automatically in the field. This paper presents a prototype device to measure chlorophyll fluorescence: the SmartFluo. The device is a combination of a smartphone offering an intuitive operation interface and an adapter implying a cuvette holder, as well as a suitable illumination source. SmartFluo is based on stimulated fluorescence of water constituents such as chlorophyll . The red band of the digital smartphone camera is sensitive enough to detect quantitatively the characteristic red fluorescence emission. The adapter contains a light source, a strong light emitting diode and additional filters to enhance the signal-to-noise ratio and to suppress the impact of scattering. A novel algorithm utilizing the red band of the camera is provided. Laboratory experiments of the SmartFluo show a linear correlation (R 2 = 0.98) to the chlorophyll concentrations measured by reference instruments, such as a high-performance benchtop laboratory fluorometer (LS 55, PerkinElmer).
为了提高内陆和沿海水域的监测能力，需要新型、价格合理、灵敏且可移动的仪器，这些仪器能够在野外半自动操作。本文介绍了一种用于测量叶绿素荧光的原型设备：SmartFluo。该设备是一部提供直观操作界面的智能手机与一个包含比色皿支架的适配器以及合适照明源的组合。SmartFluo基于叶绿素等水体成分的受激荧光原理。数字智能手机相机的红色波段灵敏度足以定量检测特征性的红色荧光发射。该适配器包含一个光源、一个强光发光二极管以及额外的滤光片，以提高信噪比并抑制散射的影响。还提供了一种利用相机红色波段的新颖算法。SmartFluo的实验室实验表明，其与参考仪器（如高性能台式实验室荧光计（LS 55，珀金埃尔默公司））测量的叶绿素浓度呈线性相关（R² = 0.98）。
