利用 CRISPR-Cas9 胞嘧啶脱氨酶融合物在水稻和番茄中的靶向碱基编辑。
Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion.
机构信息
Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Japan.
Faculty of Life and Environmental Sciences, University of Tsukuba, Gene Research Center, Tsukuba, Japan.
出版信息
Nat Biotechnol. 2017 May;35(5):441-443. doi: 10.1038/nbt.3833. Epub 2017 Mar 27.
We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.
我们将 CRISPR-Cas9 和激活诱导胞嘧啶脱氨酶（Target-AID）融合应用于通过单指导 RNA（sgRNA）在两种作物基因组区域定点突变。在水稻中，我们通过使用除草剂选择进行多重编辑诱导了多个除草剂抗性点突变，而在番茄中，我们生成了具有纯合可遗传 DNA 替换的无标记植株，证明了碱基编辑在作物改良中的可行性。
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