Stewart C M, Watson R E, Eversole L R, Fischlschweiger W, Leider A S
Department of Oral Diagnostic Sciences, University of Florida College of Dentistry, Gainesville.
Oral Surg Oral Med Oral Pathol. 1988 Apr;65(4):427-35. doi: 10.1016/0030-4220(88)90357-x.
To investigate the histogenesis of the granular cell, a large series of granular cell tumors was studied for clinical and histopathologic features with emphasis on immunocytochemical markers. The nongingival granular cell tumors (NGGCT) were found to be more prevalent among females than males by a ratio of 2:1 and arose on the tongue (67%), the buccal mucosa (13%), the lips (8%), the soft palate (6%), and other sites (6%). With the use of the avidin-biotin-peroxidase method, polyclonal rabbit antisera were employed. The antisera were directed to the following antigens: S-100 protein, myoglobin, myosin, actin, desmin, alpha-1-antitrypsin, and muramidase. Results indicated that granular cell tumors are not homogenous for immunocytochemical markers. Nongingival granular cell tumors were universally positive for S-100 protein and failed to exhibit immunoreactivity for myogenous or histiocytic markers. Alternatively, the gingival granular cell tumor of infancy was negative for all markers, whereas rhabdomyoma was reactive with myogenous markers and a subpopulation of tumor cells displayed S-100 protein immunoreactivity. The granular cell ameloblastoma was reactive only with antiserum to alpha-1-antitrypsin. Ultrastructurally, granular cells from one of two NGGCT showed a direct evolution from skeletal muscle fibers. It is concluded that the oral NGGCT is a tumor positive for S-100 protein that may arise from muscle or nerve sheath.
为了研究颗粒细胞的组织发生，我们对大量颗粒细胞瘤进行了临床和组织病理学特征研究，重点关注免疫细胞化学标记物。结果发现，非牙龈颗粒细胞瘤（NGGCT）在女性中比男性更常见，比例为2:1，好发于舌部（67%）、颊黏膜（13%）、唇部（8%）、软腭（6%）和其他部位（6%）。采用抗生物素蛋白-生物素-过氧化物酶法，使用多克隆兔抗血清。这些抗血清针对以下抗原：S-100蛋白、肌红蛋白、肌球蛋白、肌动蛋白、结蛋白、α-1抗胰蛋白酶和溶菌酶。结果表明，颗粒细胞瘤在免疫细胞化学标记物方面并非同质。非牙龈颗粒细胞瘤对S-100蛋白普遍呈阳性，对肌源性或组织细胞标记物无免疫反应性。相反，婴儿牙龈颗粒细胞瘤对所有标记物均呈阴性，而横纹肌瘤对肌源性标记物有反应，且一部分肿瘤细胞显示S-100蛋白免疫反应性。颗粒细胞成釉细胞瘤仅与抗α-1抗胰蛋白酶血清有反应。超微结构上，两个NGGCT中的一个的颗粒细胞显示出从骨骼肌纤维的直接演变。结论是，口腔NGGCT是一种S-100蛋白阳性的肿瘤，可能起源于肌肉或神经鞘。
