Bhavesh Riju, Lechuga-Vieco Ana V, Ruiz-Cabello Jesús, Herranz Fernando
Spanish Cardiovascular Research Centre (CNIC) and Spanish Pulmonary Diseases Network (CIBERES) C/Melchor Fernández-Almagro 3, 28029 Madrid, Spain.
Department of Physicochemistry II, Complutense University of Madrid, 28040 Madrid, Spain.
Nanomaterials (Basel). 2015 Nov 4;5(4):1880-1890. doi: 10.3390/nano5041880.
Iron oxide nanoparticles have long been studied as a T₂ contrast agent in MRI due to their superparamagnetic behavior. T₁-based positive contrast, being much more favorable for clinical application due to brighter and more accurate signaling is, however, still limited to gadolinium- or manganese-based imaging tools. Though being the only available commercial positive-contrast agents, they lack an efficient argument when it comes to biological toxicity and their circulatory half-life in blood. The need arises to design a biocompatible contrast agent with a scope for easy surface functionalization for long circulation in blood and/or targeted imaging. We hereby propose an extremely fast microwave synthesis for fluorescein-labeled extremely-small iron oxide nanoparticles (fdIONP), in a single step, as a viable tool for cell labeling and T₁-MRI. We demonstrate the capabilities of such an approach through high-quality magnetic resonance angiographic images of mice.
由于其超顺磁性行为，氧化铁纳米颗粒长期以来一直作为磁共振成像（MRI）中的T₂造影剂进行研究。然而，基于T₁的阳性造影剂，因其更亮且更准确的信号传递而在临床应用中更具优势，但仍仅限于基于钆或锰的成像工具。尽管它们是唯一可用的商业阳性造影剂，但在生物毒性及其在血液中的循环半衰期方面缺乏有力论据。因此，需要设计一种具有生物相容性的造影剂，该造影剂具有易于表面功能化的特点，以便在血液中长时间循环和/或进行靶向成像。在此，我们提出一种极其快速的微波合成方法，用于一步合成荧光素标记的超小氧化铁纳米颗粒（fdIONP），作为细胞标记和T₁-MRI的可行工具。我们通过小鼠的高质量磁共振血管造影图像展示了这种方法的能力。
