Zilani Md Nazmul Hasan, Sultana Tamanna, Asabur Rahman S M, Anisuzzman Md, Islam Md Amirul, Shilpi Jamil A, Hossain Md Golam
Pharmacy Discipline, Life Science School, Khulna University, Khulna, 9208, Bangladesh.
BMC Complement Altern Med. 2017 Mar 27;17(1):171. doi: 10.1186/s12906-017-1699-y.
Consumption of vegetables has been proven to be effective in the prevention of different diseases. Traditionally edible aerial part of Pisum sativum L. subsp. sativum (Fabaceae) is used to treat diabetes, heart diseases and as blood purifier. Present study was aimed to explore the traditional use of aerial parts of P. sativum as a source of antidiabetic agent. In addition, antioxidant activity and chemical composition was carried out.
Total polyphenol content was spectrophotometrically determined using Folin Chiocalteu's reagent while the flavonoids by aluminum chloride colorimetric assay. Identification of compounds of the extract was made through HPLC and LCMS. Antihyperglycemic activity was assessed by oral glucose tolerance test in mice. Antioxidant activity was determined by DPPH free radical scavenging and reducing power assay.
Total polyphenol and total flavonoids content were found to be 51.23 mg gallic acid equivalent and 30.88 mg quercetin equivalent per gram of dried plant extract respectively. Ellagic acid and p-coumeric acid were detected through HPLC. A total of eight compounds including naringenin, β-sitosterol were indentified through LCMS. In OGTT, extract (200 mg/kg bw) showed a 30.24% decrease (P< 0.05) in blood glucose levels at 30 min compared to the normal control. The extract showed IC value of 158.52 μg/mL in DPPH scavenging assay and also showed comparable reducing power.
Along with other compounds ellagic acid and β-sitosterol present in the extract may be responsible for its antioxidant as well as antihyperglycemic activities. Altogether these results rationalize the use of this vegetable in traditional medicine.
食用蔬菜已被证明对预防多种疾病有效。传统上，豌豆（豆科豌豆属豌豆亚种）的可食用地上部分用于治疗糖尿病、心脏病，并作为血液净化剂。本研究旨在探索豌豆地上部分作为抗糖尿病药物来源的传统用途。此外，还进行了抗氧化活性和化学成分分析。
使用福林 - 肖卡泰试剂通过分光光度法测定总多酚含量，通过氯化铝比色法测定黄酮类化合物含量。提取物中的化合物通过高效液相色谱法（HPLC）和液相色谱 - 质谱联用（LCMS）进行鉴定。通过小鼠口服葡萄糖耐量试验评估降血糖活性。通过二苯基苦味酰基自由基（DPPH）清除试验和还原能力测定来确定抗氧化活性。
每克干燥植物提取物中总多酚和总黄酮含量分别为51.23毫克没食子酸当量和30.88毫克槲皮素当量。通过HPLC检测到鞣花酸和对香豆酸。通过LCMS共鉴定出包括柚皮素、β - 谷甾醇在内的8种化合物。在口服葡萄糖耐量试验中，与正常对照组相比，提取物（200毫克/千克体重）在30分钟时血糖水平降低了30.24%（P < 0.05）。提取物在DPPH清除试验中的IC值为158.52微克/毫升，并且还表现出相当的还原能力。
提取物中存在的鞣花酸和β - 谷甾醇等其他化合物可能是其抗氧化和降血糖活性的原因。总之，这些结果证明了这种蔬菜在传统医学中的应用合理性。
