Promotion Center for Global Materials Research (PCGMR), Department of Material Science and Engineering, National Cheng Kung University, Tainan, Taiwan.
Applied Chemistry, Chaoyang University of Technology, Taichung, Taiwan.
Spectrochim Acta A Mol Biomol Spectrosc. 2017 Jun 15;181:91-97. doi: 10.1016/j.saa.2017.03.040. Epub 2017 Mar 21.
A novel, low-cost and biocompatible three-dimensional (3D) substrate for surface-enhanced Raman spectroscopy (SERS) is fabricated using gold nanoparticles (AuNPs) loaded on cellulose paper for detection of amino acids and melamine. Dysosma pleiantha rhizome (Dp-Rhi) capped AuNPs (Dp-Rhi_AuNPs) were prepared by in situ using aqueous extract of Dp-Rhi and in situ functionalized Dp-Rhi on AuNPs surface was verified by Fourier transform infrared spectroscopy and zeta potentials analysis shows a negative (-18.4mV) surface charges, which confirm that presence of Dp-Rhi on AuNPs. The biocompatibility of Dp-Rhi_AuNPs is also examined by cell viability of FaDu cells using MTS assay and compared to control group. In conclusion, the SERS performance of AuNPs@cellulose paper substrates were systematically demonstrated and examined with different excitation wavelengths (i.e. 532, 632.8 and 785nm lasers) and the as-prepared 3D substrates provided an enhancement factor approaching 7 orders of magnitude compared with conventional Raman intensity using para-nitrothiophenol (p-NTP), para-aminothiophenol (p-ATP) and para-mercaptobenzoic acid (p-MBA) as probe molecules. The strong electromagnetic effect was generated at the interface of AuNPs and pre-treated roughened cellulose paper is also investigated by simulation in which the formation of possible Raman hot-spot zone in fiber-like microstructure of cellulose paper decorated with AuNPs. Notably, with optimized condition of as-prepared 3D AuNPs@cellulose paper is highly sensitive in the SERS detection of aqueous tyrosine (10M) and melamine (10M).
一种新型的、低成本的、生物兼容的三维（3D）基底，用于表面增强拉曼光谱（SERS），是通过将负载在纤维素纸上的金纳米粒子（AuNPs）制造的，用于检测氨基酸和三聚氰胺。通过原位使用 Dp-Rhi 的水提物制备了 Dp-Rhi 封端的 AuNPs（Dp-Rhi_AuNPs），并通过傅里叶变换红外光谱和 zeta 电位分析验证了 AuNPs 表面的原位功能化，结果表明表面带有负电荷（-18.4mV），这证实了 Dp-Rhi 存在于 AuNPs 上。通过 MTS 测定法检测 FaDu 细胞的细胞活力，还研究了 Dp-Rhi_AuNPs 的生物相容性，并与对照组进行了比较。总之，系统地展示和研究了 AuNPs@纤维素纸基底的 SERS 性能，使用不同的激发波长（即 532、632.8 和 785nm 激光）进行了研究，与使用对硝基硫代苯酚（p-NTP）、对氨基硫代苯酚（p-ATP）和对巯基苯甲酸（p-MBA）作为探针分子的常规拉曼强度相比，所制备的 3D 基底提供了接近 7 个数量级的增强因子。还通过模拟研究了 AuNPs 和预处理粗糙化纤维素纸界面处的强电磁效应，其中模拟了 AuNPs 修饰的纤维素纸纤维状微观结构中可能形成的 Raman 热点区域。值得注意的是，在所制备的 3D AuNPs@纤维素纸的优化条件下，对水溶液中天冬氨酸（10M）和三聚氰胺（10M）的 SERS 检测具有很高的灵敏度。
