Lu Yongxin, Luo Yan, Lin Zehao, Huang Jianguo
Department of Chemistry, Zhejiang University, Hangzhou, Zhejiang 310027, P. R. China.
Shaoxing Test Institute of Quality and Technical Supervision, Shaoxing, Zhejiang 312071, P. R. China.
Beilstein J Nanotechnol. 2019 Jun 24;10:1270-1279. doi: 10.3762/bjnano.10.126. eCollection 2019.
A highly active surface-enhanced Raman scattering (SERS) substrate was developed by facile deposition of silver nanoparticles onto cellulose fibers of ordinary laboratory filter paper. This was achieved by means of the silver mirror reaction in a manner to control both the size of the silver nanoparticles and the silver density of the substrate. This paper-based substrate is composed of a particle-on-fiber structure with the unique three-dimensional network morphology of the cellulose matrix. For such a SERS substrate with optimized size of the silver nanoparticles (ca. 70 nm) and loading density of silver (17.28 wt %), a remarkable detection limit down to the sub-attomolar (1 × 10 M) level and an enhancement factor of 3 × 10 were achieved by using Rhodamine 6G as the analyte. Moreover, this substrate was applied to monitor the molecular recognition through multiple hydrogen bonds in between nucleosides of adenosine and thymidine. This low-cost, highly sensitive, and biocompatible paper-based SERS substrate holds considerable potentials for the detection and analyses of chemical and biomolecular species.
通过将银纳米颗粒轻松沉积到普通实验室滤纸的纤维素纤维上，制备了一种高活性的表面增强拉曼散射（SERS）基底。这是通过银镜反应实现的，以此来控制银纳米颗粒的尺寸和基底的银密度。这种纸基基底由纤维上颗粒结构组成，具有纤维素基质独特的三维网络形态。对于这种银纳米颗粒尺寸优化（约70 nm）且银负载密度为17.28 wt%的SERS基底，以罗丹明6G作为分析物时，实现了低至亚阿托摩尔（1×10⁻¹⁸ M）水平的显著检测限和3×10⁶的增强因子。此外，该基底被用于监测腺苷和胸腺嘧啶核苷之间通过多个氢键的分子识别。这种低成本、高灵敏度且具有生物相容性的纸基SERS基底在化学和生物分子物种的检测与分析方面具有巨大潜力。
