一种用于无介体 NADH 传感和生物传感的透明质酸分散碳纳米管电极。

A hyaluronic acid dispersed carbon nanotube electrode used for a mediatorless NADH sensing and biosensing.

机构信息

Department of Glycobiotechnology, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava, SK-845 38, Slovakia.

出版信息

Talanta. 2011 Apr 15;84(2):355-61. doi: 10.1016/j.talanta.2011.01.004. Epub 2011 Jan 15.

DOI:10.1016/j.talanta.2011.01.004

PMID:21376957

Abstract

A biocompatible nanocomposite consisting of single-walled carbon nanotubes (CNTs) dispersed in a hyaluronic acid (HA) was investigated as a sensing platform for a mediatorless electrochemical detection of NADH. The device was characterised by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and extensively by electrochemistry. CNT-HA bionanocomposite showed more reversible electrochemistry, higher short-term stability of NADH sensing and higher selectivity of NADH detection compared to frequently used CNT-CHI (chitosan) modified GCE. Finally the performance of the sensor modified by CNT-HA was tested in a batch and flow injection analysis (FIA) mode of operation with basic characteristics revealed. The NADH sensor exhibits a good long-term operational stability (95% of the original sensitivity after 22 h of continuous operation). Subsequently a d-sorbitol biosensor based on such a nanoscale built interface was prepared and characterised with a d-sorbitol dehydrogenase used as a biocatalyst.

摘要

一种由单壁碳纳米管（CNT）分散在透明质酸（HA）中的生物相容性纳米复合材料被研究作为一种无介体电化学检测 NADH 的传感平台。该器件通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）和电化学方法进行了广泛的表征。与常用的 CNT-CHI（壳聚糖）修饰的 GCE 相比，CNT-HA 纳米复合材料具有更可逆的电化学、更高的 NADH 传感短期稳定性和更高的 NADH 检测选择性。最后，通过 CNT-HA 修饰的传感器在批处理和流动注射分析（FIA）操作模式下进行了性能测试，揭示了其基本特性。NADH 传感器具有良好的长期运行稳定性（连续运行 22 小时后，原始灵敏度的 95%）。随后，基于这种纳米级构建界面，制备了一种 d-山梨醇生物传感器，并使用 d-山梨醇脱氢酶作为生物催化剂对其进行了表征。