• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于短单链和双链DNA在氧化石墨烯上吸附动力学差异的分离

Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide.

作者信息

Huang Po-Jung Jimmy, Liu Juewen

机构信息

Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.

出版信息

Nanomaterials (Basel). 2013 Apr 4;3(2):221-228. doi: 10.3390/nano3020221.

DOI:10.3390/nano3020221
PMID:28348332
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5327888/
Abstract

Separation of short single- and double-stranded DNA typically requires gel electrophoresis followed by DNA extraction, which is a time consuming process. Graphene oxide adsorbs single-stranded DNA more quickly than double-stranded ones, allowing for selective removal of the former with a simple mixing and centrifugation operation. The effect of DNA length and salt on adsorption selectivity has been characterized and its application in DNA melting curve measurement has been demonstrated.

摘要

短单链和双链DNA的分离通常需要先进行凝胶电泳，然后进行DNA提取，这是一个耗时的过程。氧化石墨烯吸附单链DNA的速度比双链DNA更快，通过简单的混合和离心操作就可以选择性地去除前者。已经对DNA长度和盐对吸附选择性的影响进行了表征，并证明了其在DNA熔解曲线测量中的应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0368/5327888/7fd0c7808061/nanomaterials-03-00221-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0368/5327888/dd66c6cd67a6/nanomaterials-03-00221-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0368/5327888/230c8db9e309/nanomaterials-03-00221-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0368/5327888/83a55e30f254/nanomaterials-03-00221-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0368/5327888/7fd0c7808061/nanomaterials-03-00221-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0368/5327888/dd66c6cd67a6/nanomaterials-03-00221-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0368/5327888/230c8db9e309/nanomaterials-03-00221-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0368/5327888/83a55e30f254/nanomaterials-03-00221-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0368/5327888/7fd0c7808061/nanomaterials-03-00221-g004.jpg

相似文献

1
Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide.基于短单链和双链DNA在氧化石墨烯上吸附动力学差异的分离
Nanomaterials (Basel). 2013 Apr 4;3(2):221-228. doi: 10.3390/nano3020221.
2
Effect of pH and Salt on Adsorption of Double-Stranded DNA on Graphene Oxide.pH值和盐对双链DNA在氧化石墨烯上吸附的影响。
J Nanosci Nanotechnol. 2015 Oct;15(10):7913-7. doi: 10.1166/jnn.2015.11217.
3
Desorption of single-stranded nucleic acids from graphene oxide by disruption of hydrogen bonding.通过破坏氢键将单链核酸从氧化石墨烯上解吸。
Analyst. 2013 Mar 21;138(6):1745-9. doi: 10.1039/c3an36493c. Epub 2013 Jan 30.
4
Fast and simple method for screening of single-stranded DNA breaking photosensitizers using graphene oxide.使用氧化石墨烯筛选单链DNA断裂光敏剂的快速简便方法。
Nano Converg. 2018 Oct 22;5(1):29. doi: 10.1186/s40580-018-0160-3.
5
Unidirectional pulsed-field electrophoresis of single- and double-stranded DNA in agarose gels: analytical expressions relating mobility and molecular length and their application in the measurement of strand breaks.琼脂糖凝胶中单链和双链DNA的单向脉冲场电泳：迁移率与分子长度的解析表达式及其在链断裂测量中的应用
Anal Biochem. 1987 May 1;162(2):511-20. doi: 10.1016/0003-2697(87)90427-1.
6
Adsorption and desorption of DNA on graphene oxide studied by fluorescently labeled oligonucleotides.通过荧光标记寡核苷酸研究 DNA 在氧化石墨烯上的吸附和解吸。
Langmuir. 2011 Mar 15;27(6):2731-8. doi: 10.1021/la1037926. Epub 2011 Feb 8.
7
Mechanism of DNA adsorption and desorption on graphene oxide.DNA在氧化石墨烯上的吸附和解吸机制。
Langmuir. 2014 Oct 28;30(42):12587-95. doi: 10.1021/la503401d. Epub 2014 Oct 15.
8
[Effect of single-stranded and double-stranded breaks on the melting temperature of phage T2 DNA].[单链和双链断裂对噬菌体T2 DNA解链温度的影响]
Mol Biol (Mosk). 1979 May-Jun;13(3):531-42.
9
Electrochemical label-free and sensitive nanobiosensing of DNA hybridization by graphene oxide modified pencil graphite electrode.氧化石墨烯修饰铅笔石墨电极用于DNA杂交的无电化学标记灵敏纳米生物传感
Anal Biochem. 2017 Sep 1;532:64-71. doi: 10.1016/j.ab.2017.06.004. Epub 2017 Jun 9.
10
Responsive polymer gels: double-stranded versus single-stranded DNA.响应性聚合物凝胶：双链DNA与单链DNA
J Phys Chem B. 2007 Sep 20;111(37):10886-96. doi: 10.1021/jp0713108. Epub 2007 Aug 30.

引用本文的文献

1
Magnetic Graphene Oxide Nanocomposites for Selective miRNA Separation and Recovery.用于选择性分离和回收微小RNA的磁性氧化石墨烯纳米复合材料
ACS Omega. 2024 Jan 2;9(2):2263-2271. doi: 10.1021/acsomega.3c05919. eCollection 2024 Jan 16.
2
Biosensing Systems Based on Graphene Oxide Fluorescence Quenching Effect.基于氧化石墨烯荧光猝灭效应的生物传感系统
Micromachines (Basel). 2023 Jul 28;14(8):1522. doi: 10.3390/mi14081522.
3
Graphene Oxide/Nitrocellulose Non-Covalent Hybrid as Solid Phase for Oligo-DNA Extraction from Complex Medium.

本文引用的文献

1
Desorption of single-stranded nucleic acids from graphene oxide by disruption of hydrogen bonding.通过破坏氢键将单链核酸从氧化石墨烯上解吸。
Analyst. 2013 Mar 21;138(6):1745-9. doi: 10.1039/c3an36493c. Epub 2013 Jan 30.
2
Graphene oxide: preparation, functionalization, and electrochemical applications.氧化石墨烯：制备、功能化及电化学应用。
Chem Rev. 2012 Nov 14;112(11):6027-53. doi: 10.1021/cr300115g. Epub 2012 Aug 14.
3
Adsorption of DNA onto gold nanoparticles and graphene oxide: surface science and applications.金纳米粒子和氧化石墨烯上 DNA 的吸附：表面科学与应用。
氧化石墨烯/硝化纤维素非共价杂化体作为从复杂介质中提取寡脱氧核苷酸的固相。
Molecules. 2023 Jun 7;28(12):4599. doi: 10.3390/molecules28124599.
4
Rapid and Sensitive Detection of Antibiotic Resistance Genes by Utilizing TALEs as a Diagnostic Probe with 2D-Nanosheet Graphene Oxide.利用 TALEs 作为诊断探针结合 2D 纳米片石墨烯氧化物快速灵敏检测抗生素耐药基因
Anal Chem. 2023 Jun 27;95(25):9505-9512. doi: 10.1021/acs.analchem.3c00647. Epub 2023 Jun 13.
5
Thrombin Determination Using Graphene Oxide Sensors with Co-Assisted Amplification.使用具有协同辅助放大作用的氧化石墨烯传感器测定凝血酶
Micromachines (Basel). 2022 Aug 31;13(9):1435. doi: 10.3390/mi13091435.
6
Graphene-Based Strategies in Liquid Biopsy and in Viral Diseases Diagnosis.基于石墨烯的液体活检及病毒疾病诊断策略
Nanomaterials (Basel). 2020 May 26;10(6):1014. doi: 10.3390/nano10061014.
7
Nanomaterial-based optical and electrochemical techniques for detection of methicillin-resistant Staphylococcus aureus: a review.基于纳米材料的光学和电化学技术检测耐甲氧西林金黄色葡萄球菌：综述。
Mikrochim Acta. 2019 Jan 16;186(2):114. doi: 10.1007/s00604-018-3186-7.
8
A fluorescent DNA based probe for Hg(II) based on thymine-Hg(II)-thymine interaction and enrichment via magnetized graphene oxide.基于胸腺嘧啶-Hg(II)-胸腺嘧啶相互作用的荧光 DNA 探针用于 Hg(II)的检测，并通过磁性氧化石墨烯进行富集。
Mikrochim Acta. 2018 Mar 3;185(3):207. doi: 10.1007/s00604-018-2689-6.
9
Universal sensor array for highly selective system identification using two-dimensional nanoparticles.用于使用二维纳米颗粒进行高选择性系统识别的通用传感器阵列。
Chem Sci. 2017 Aug 1;8(8):5735-5745. doi: 10.1039/c7sc01522d. Epub 2017 Jun 16.
10
Nanomaterials in Sensors.传感器中的纳米材料。
Nanomaterials (Basel). 2013 Oct 14;3(4):572-573. doi: 10.3390/nano3040572.
Phys Chem Chem Phys. 2012 Aug 14;14(30):10485-96. doi: 10.1039/c2cp41186e. Epub 2012 Jun 28.
4
Capture of double-stranded DNA in stacked-graphene: giving new insight into the graphene/DNA interaction.双层 DNA 在堆叠石墨烯中的捕获：深入了解石墨烯/DNA 相互作用。
Chem Commun (Camb). 2012 Jan 14;48(4):564-6. doi: 10.1039/c1cc16429e. Epub 2011 Nov 8.
5
A graphene oxide platform for the assay of biomolecules based on chemiluminescence resonance energy transfer.基于化学发光共振能量转移的用于生物分子检测的氧化石墨烯平台。
Chem Commun (Camb). 2012 Jan 4;48(1):106-8. doi: 10.1039/c1cc15443e. Epub 2011 Oct 31.
6
Adsorption of double-stranded DNA to graphene oxide preventing enzymatic digestion.氧化石墨烯阻止双链 DNA 的酶解吸附
Nanoscale. 2011 Sep 1;3(9):3888-92. doi: 10.1039/c1nr10617a. Epub 2011 Aug 9.
7
Adsorption and desorption of DNA on graphene oxide studied by fluorescently labeled oligonucleotides.通过荧光标记寡核苷酸研究 DNA 在氧化石墨烯上的吸附和解吸。
Langmuir. 2011 Mar 15;27(6):2731-8. doi: 10.1021/la1037926. Epub 2011 Feb 8.
8
Graphene oxide as a chemically tunable platform for optical applications.氧化石墨烯：用于光学应用的化学可调谐平台。
Nat Chem. 2010 Dec;2(12):1015-24. doi: 10.1038/nchem.907. Epub 2010 Nov 23.
9
Comparison of methods for accurate quantification of DNA mass concentration with traceability to the international system of units.比较具有国际单位制溯源性的准确测量 DNA 质量浓度的方法。
Anal Chem. 2010 Sep 1;82(17):7185-92. doi: 10.1021/ac100845m.
10
Constraint of DNA on functionalized graphene improves its biostability and specificity.DNA对功能化石墨烯的约束提高了其生物稳定性和特异性。
Small. 2010 Jun 6;6(11):1205-9. doi: 10.1002/smll.201000024.