Sagoh T, Yamada M
Institute for Protein Research, Osaka University, Japan.
Arch Biochem Biophys. 1988 May 1;262(2):599-604. doi: 10.1016/0003-9861(88)90411-0.
Myeloperoxidase gene transcription in isolated nuclei from HL-60 cells induced to differentiate into granulocytes by dimethyl sulfoxide or into macrophages by 12-O-tetradecanoylphorbol-13-acetate was studied by dot-blot hybridization of a myeloperoxidase cDNA to the 32P-labeled nuclear transcripts. Myeloperoxidase gene transcription, like that of c-myc gene transcription, was reduced to a low level within 12 h after the inductions of these differentiations. In contrast, transcription of the actin gene remained constant. These results indicate that decrease in myeloperoxidase synthesis in HL-60 cells during differentiation is regulated at a transcriptional level.
通过用髓过氧化物酶cDNA与32P标记的核转录本进行斑点杂交，研究了用二甲基亚砜诱导HL-60细胞分化为粒细胞或用12-O-十四烷酰佛波醇-13-乙酸酯诱导其分化为巨噬细胞后，分离细胞核中的髓过氧化物酶基因转录情况。髓过氧化物酶基因转录，与c-myc基因转录一样，在这些分化诱导后的12小时内降至低水平。相比之下，肌动蛋白基因的转录保持恒定。这些结果表明，HL-60细胞在分化过程中髓过氧化物酶合成的减少是在转录水平上受到调控的。
