• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

3' 尿苷酸化在CD4 T细胞活化过程中控制成熟微小RNA的周转。

3' Uridylation controls mature microRNA turnover during CD4 T-cell activation.

作者信息

Gutiérrez-Vázquez Cristina, Enright Anton J, Rodríguez-Galán Ana, Pérez-García Arantxa, Collier Paul, Jones Matthew R, Benes Vladimir, Mizgerd Joseph P, Mittelbrunn María, Ramiro Almudena R, Sánchez-Madrid Francisco

机构信息

Instituto de Investigación Sanitaria Princesa, Hospital Universitario de la Princesa, Universidad Autónoma de Madrid, Madrid 28006, Spain.

Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid 28029, Spain.

出版信息

RNA. 2017 Jun;23(6):882-891. doi: 10.1261/rna.060095.116. Epub 2017 Mar 28.

DOI:10.1261/rna.060095.116
PMID:28351886
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5435861/
Abstract

Activation of T lymphocytes requires a tight regulation of microRNA (miRNA) expression. Terminal uridyltransferases (TUTases) catalyze 3' nontemplated nucleotide addition (3'NTA) to miRNAs, which may influence miRNA stability and function. Here, we investigated 3'NTA to mature miRNA in CD4 T lymphocytes by deep sequencing. Upon T-cell activation, miRNA sequences bearing terminal uridines are specifically decreased, concomitantly with down-regulation of TUT4 and TUT7 enzymes. Analyzing TUT4-deficient T lymphocytes, we proved that this terminal uridyltransferase is essential for the maintenance of miRNA uridylation in the steady state of T lymphocytes. Analysis of synthetic uridylated miRNAs shows that 3' addition of uridine promotes degradation of these uridylated miRNAs after T-cell activation. Our data underline post-transcriptional uridylation as a mechanism to fine-tune miRNA levels during T-cell activation.

摘要

T淋巴细胞的激活需要对微小RNA（miRNA）表达进行严格调控。末端尿苷转移酶（TUTases）催化miRNA的3'非模板化核苷酸添加（3'NTA），这可能影响miRNA的稳定性和功能。在此，我们通过深度测序研究了CD4 T淋巴细胞中成熟miRNA的3'NTA情况。在T细胞激活后，带有末端尿苷的miRNA序列特异性减少，同时TUT4和TUT7酶的表达下调。通过分析TUT4缺陷型T淋巴细胞，我们证明这种末端尿苷转移酶对于T淋巴细胞稳态下miRNA尿苷化的维持至关重要。对合成的尿苷化miRNA的分析表明，尿苷的3'添加促进了T细胞激活后这些尿苷化miRNA的降解。我们的数据强调转录后尿苷化是T细胞激活过程中微调miRNA水平的一种机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff4c/5435861/d7fd052324bd/882f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff4c/5435861/57bce0737649/882f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff4c/5435861/2a3fce708298/882f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff4c/5435861/686a0f63e592/882f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff4c/5435861/d7fd052324bd/882f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff4c/5435861/57bce0737649/882f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff4c/5435861/2a3fce708298/882f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff4c/5435861/686a0f63e592/882f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff4c/5435861/d7fd052324bd/882f04.jpg

相似文献

1
3' Uridylation controls mature microRNA turnover during CD4 T-cell activation.3' 尿苷酸化在CD4 T细胞活化过程中控制成熟微小RNA的周转。
RNA. 2017 Jun;23(6):882-891. doi: 10.1261/rna.060095.116. Epub 2017 Mar 28.
2
Selective microRNA uridylation by Zcchc6 (TUT7) and Zcchc11 (TUT4).由Zcchc6（TUT7）和Zcchc11（TUT4）介导的选择性微小核糖核酸尿苷化
Nucleic Acids Res. 2014 Oct;42(18):11777-91. doi: 10.1093/nar/gku805. Epub 2014 Sep 15.
3
TUT7 controls the fate of precursor microRNAs by using three different uridylation mechanisms.TUT7通过三种不同的尿苷化机制控制前体微小RNA的命运。
EMBO J. 2015 Jul 2;34(13):1801-15. doi: 10.15252/embj.201590931. Epub 2015 May 15.
4
3' Uridylation Confers miRNAs with Non-canonical Target Repertoires.3' 尿嘧啶酰化赋予 miRNA 非经典靶基因谱。
Mol Cell. 2019 Aug 8;75(3):511-522.e4. doi: 10.1016/j.molcel.2019.05.014. Epub 2019 Jun 6.
5
Uridylation by TUT4 and TUT7 marks mRNA for degradation.TUT4和TUT7介导的尿苷酸化标记mRNA以便降解。
Cell. 2014 Dec 4;159(6):1365-76. doi: 10.1016/j.cell.2014.10.055.
6
RNA uridyl transferases TUT4/7 differentially regulate miRNA variants depending on the cancer cell type.RNA 尿嘧啶转移酶 TUT4/7 根据癌细胞类型差异调控 miRNA 变体。
RNA. 2022 Mar;28(3):353-370. doi: 10.1261/rna.078976.121. Epub 2021 Dec 23.
7
Zcchc11 uridylates mature miRNAs to enhance neonatal IGF-1 expression, growth, and survival.Zcchc11 使成熟的 miRNA 发生尿嘧啶化，从而增强新生 IGF-1 的表达、促进生长和提高存活率。
PLoS Genet. 2012;8(11):e1003105. doi: 10.1371/journal.pgen.1003105. Epub 2012 Nov 29.
8
3' RNA Uridylation in Epitranscriptomics, Gene Regulation, and Disease.表观转录组学、基因调控及疾病中的3'端RNA尿苷酸化
Front Mol Biosci. 2018 Jul 13;5:61. doi: 10.3389/fmolb.2018.00061. eCollection 2018.
9
Uridylation by TUT4/7 Restricts Retrotransposition of Human LINE-1s.TUT4/7 的尿嘧啶化作用限制了人类 LINE-1 逆转录转座。
Cell. 2018 Sep 6;174(6):1537-1548.e29. doi: 10.1016/j.cell.2018.07.022. Epub 2018 Aug 16.
10
Identification of small molecule inhibitors of Zcchc11 TUTase activity.Zcchc11末端尿苷转移酶（TUTase）活性小分子抑制剂的鉴定。
RNA Biol. 2015;12(8):792-800. doi: 10.1080/15476286.2015.1058478.

引用本文的文献

1
Post-Transcriptional Modifications to miRNAs Undergo Widespread Alterations, Creating a Unique Lung Adenocarcinoma IsomiRome.微小RNA的转录后修饰经历广泛改变，形成独特的肺腺癌异构体组。
Cancers (Basel). 2024 Sep 28;16(19):3322. doi: 10.3390/cancers16193322.
2
MBNL splicing factors regulate the microtranscriptome of skeletal muscles.MBNL 剪接因子调节骨骼肌的微转录组。
Nucleic Acids Res. 2024 Oct 28;52(19):12055-12073. doi: 10.1093/nar/gkae774.
3
What goes up must come down: off switches for regulatory RNAs.上行下效：调控 RNA 的关闭开关。

本文引用的文献

1
TUT7 catalyzes the uridylation of the 3' end for rapid degradation of histone mRNA.TUT7催化组蛋白mRNA 3'端的尿苷酸化，使其快速降解。
RNA. 2016 Nov;22(11):1673-1688. doi: 10.1261/rna.058107.116. Epub 2016 Sep 8.
2
Uridylation Earmarks mRNAs for Degradation… and More.尿苷酰化标记 mRNA 进行降解……等等。
Trends Genet. 2016 Oct;32(10):607-619. doi: 10.1016/j.tig.2016.08.003. Epub 2016 Sep 1.
3
Uridylation and PABP Cooperate to Repair mRNA Deadenylated Ends in Arabidopsis.尿苷化与多聚腺苷酸结合蛋白协同修复拟南芥中去腺苷酸化的mRNA末端。
Genes Dev. 2024 Aug 20;38(13-14):597-613. doi: 10.1101/gad.351934.124.
4
Small RNAs: An expanding world with therapeutic promises.小RNA：一个充满治疗前景的不断扩展的领域。
Fundam Res. 2023 Apr 6;3(5):676-682. doi: 10.1016/j.fmre.2023.03.003. eCollection 2023 Sep.
5
Interrogating the Role of miR-125b and Its 3'isomiRs in Protection against Hypoxia.探讨 miR-125b 及其 3'异构体在缺氧保护中的作用。
Int J Mol Sci. 2023 Nov 6;24(21):16015. doi: 10.3390/ijms242116015.
6
Identification of microRNA editing sites in clear cell renal cell carcinoma.鉴定肾透明细胞癌中的 microRNA 编辑位点。
Sci Rep. 2023 Sep 13;13(1):15117. doi: 10.1038/s41598-023-42302-y.
7
ISG20L2: an RNA nuclease regulating T cell activation.ISG20L2：一种调节 T 细胞激活的 RNA 核酸酶。
Cell Mol Life Sci. 2023 Aug 30;80(9):273. doi: 10.1007/s00018-023-04925-2.
8
RNA Metabolism in T Lymphocytes.T淋巴细胞中的RNA代谢
Immune Netw. 2022 Oct 7;22(5):e39. doi: 10.4110/in.2022.22.e39. eCollection 2022 Oct.
9
RNA modifications: importance in immune cell biology and related diseases.RNA 修饰：在免疫细胞生物学和相关疾病中的重要性。
Signal Transduct Target Ther. 2022 Sep 22;7(1):334. doi: 10.1038/s41392-022-01175-9.
10
Natural killer (NK) cell-derived extracellular-vesicle shuttled microRNAs control T cell responses.自然杀伤 (NK) 细胞衍生的细胞外囊泡转导的 microRNAs 调控 T 细胞反应。
Elife. 2022 Jul 29;11:e76319. doi: 10.7554/eLife.76319.
Cell Rep. 2016 Mar 22;14(11):2707-17. doi: 10.1016/j.celrep.2016.02.060. Epub 2016 Mar 10.
4
Identification of cytokine-induced modulation of microRNA expression and secretion as measured by a novel microRNA specific qPCR assay.通过一种新型的微小RNA特异性定量聚合酶链反应检测法鉴定细胞因子诱导的微小RNA表达和分泌的调节作用。
Sci Rep. 2015 Jun 25;5:11590. doi: 10.1038/srep11590.
5
Uridylation by TUT4 and TUT7 marks mRNA for degradation.TUT4和TUT7介导的尿苷酸化标记mRNA以便降解。
Cell. 2014 Dec 4;159(6):1365-76. doi: 10.1016/j.cell.2014.10.055.
6
Selective microRNA uridylation by Zcchc6 (TUT7) and Zcchc11 (TUT4).由Zcchc6（TUT7）和Zcchc11（TUT4）介导的选择性微小核糖核酸尿苷化
Nucleic Acids Res. 2014 Oct;42(18):11777-91. doi: 10.1093/nar/gku805. Epub 2014 Sep 15.
7
miRBase: annotating high confidence microRNAs using deep sequencing data.miRBase：利用深度测序数据注释高可信度 microRNAs。
Nucleic Acids Res. 2014 Jan;42(Database issue):D68-73. doi: 10.1093/nar/gkt1181. Epub 2013 Nov 25.
8
Cytoplasmic RNA: a case of the tail wagging the dog.细胞质 RNA：一种本末倒置的情况。
Nat Rev Mol Cell Biol. 2013 Oct;14(10):643-53. doi: 10.1038/nrm3645. Epub 2013 Aug 29.
9
MicroRNA-mediated regulation of T helper cell differentiation and plasticity.miRNA 介导的辅助性 T 细胞分化和可塑性调节。
Nat Rev Immunol. 2013 Sep;13(9):666-78. doi: 10.1038/nri3494. Epub 2013 Aug 2.
10
Kraken: a set of tools for quality control and analysis of high-throughput sequence data.Kraken：一组用于高通量测序数据质量控制和分析的工具。
Methods. 2013 Sep 1;63(1):41-9. doi: 10.1016/j.ymeth.2013.06.027. Epub 2013 Jun 29.