Han Jin, Suga Keishi, Hayashi Keita, Okamoto Yukihiro, Umakoshi Hiroshi
Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University , 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
Department of Chemical Engineering, National Institute of Technology , Nara College, 22 Yata-cho, Yamatokoriyama, Nara 639-1080, Japan.
J Phys Chem B. 2017 Apr 27;121(16):4091-4098. doi: 10.1021/acs.jpcb.7b00368. Epub 2017 Apr 10.
Resveratrol (RES) is a type of polyphenolic compound discovered from grapes and has gained prominence as a possible contributor to many disease treatments. Herein, three different types of liposomes were prepared as model cell membranes, and then the influence of the incorporation of RES on their membrane properties was evaluated by utilizing membrane-binding fluorescent probes. The binding of RES lead to the membrane polarities decreasing slightly, regardless of the phase states of the membrane, while the membrane fluidities decreased only in the case of liquid-disordered phase. In each model membrane system, the incorporation of RES dramatically dehydrated the membrane surface, which could prevent the permeation of water-soluble materials. Fluorescence quenching of Laurdan indicated less accessibility of hydroxyl radial into the inner region of the RES-incorporated membrane. The comparison between the mass spectra of oxidized DOPC molecules treated with hydroxyl radical revealed that the RES-incorporation into DOPC membranes can contribute to prevent lipid oxidation. It is concluded that the binding of RES to the lipid membrane can play a key role in affecting membrane properties and functions.
白藜芦醇（RES）是一种从葡萄中发现的多酚类化合物，作为许多疾病治疗的潜在贡献者而备受关注。在此，制备了三种不同类型的脂质体作为模型细胞膜，然后利用膜结合荧光探针评估了RES掺入对其膜性质的影响。RES的结合导致膜极性略有下降，无论膜的相态如何，而膜流动性仅在液晶无序相的情况下下降。在每个模型膜系统中，RES的掺入使膜表面显著脱水，这可以防止水溶性物质的渗透。劳丹的荧光猝灭表明羟基自由基进入掺入RES的膜内部区域的可及性较低。用羟基自由基处理的氧化DOPC分子的质谱比较表明，RES掺入DOPC膜有助于防止脂质氧化。得出的结论是，RES与脂质膜的结合在影响膜性质和功能方面可以发挥关键作用。
