Jenison S A, Firzlaff J M, Langenberg A, Galloway D A
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
J Virol. 1988 Jun;62(6):2115-23. doi: 10.1128/JVI.62.6.2115-2123.1988.
Human papillomavirus (HPVs) infect the genital epithelium and are found in proliferative lesions ranging from benign condylomata to invasive carcinomas. The immunological response to these infections is poorly understood because of the lack of purified viral antigens. In this study, bacterially derived fusion proteins expressing segments of all the major open reading frames (ORFs) of HPV type 6b (HPV-6b) have been used in Western blot (immunoblot) assays to detect antibodies directed against HPV-encoded proteins. The most striking reactivities present in sera from patients with genital warts were to the HPV-6b L1 ORF protein and, to a lesser extent, to the HPV-6b L2 ORF protein. Two cases of reactivity to HPV-6b E2 ORF were observed, but no reactivities were seen with other HPV-6b constructs. Two sera reacted with the HPV-16 L2 fusion protein, and two sera reacted with the HPV-16 E4 protein. The antibodies directed against the HPV-6b fusion proteins showed no cross-reactivity with comparable regions of the HPV-16 ORFs. This assay provides a useful approach for further studies of HPV serology.
人乳头瘤病毒（HPV）感染生殖上皮，存在于从良性湿疣到浸润性癌的增殖性病变中。由于缺乏纯化的病毒抗原，对这些感染的免疫反应了解甚少。在本研究中，表达6b型人乳头瘤病毒（HPV - 6b）所有主要开放阅读框（ORF）片段的细菌衍生融合蛋白已用于蛋白质印迹（免疫印迹）分析，以检测针对HPV编码蛋白的抗体。尖锐湿疣患者血清中最显著的反应性针对HPV - 6b L1 ORF蛋白，其次是针对HPV - 6b L2 ORF蛋白。观察到两例对HPV - 6b E2 ORF的反应性，但与其他HPV - 6b构建体未见反应性。两份血清与HPV - 16 L2融合蛋白反应，两份血清与HPV - 16 E4蛋白反应。针对HPV - 6b融合蛋白的抗体与HPV - 16 ORF的可比区域无交叉反应。该分析为HPV血清学的进一步研究提供了一种有用的方法。
