尖锐湿疣患者宫颈分泌物中抗乳头瘤病毒抗原的抗体。
Antibodies against papillomavirus antigens in cervical secretions from condyloma patients.
作者信息
Dillner L, Fredriksson A, Persson E, Forslund O, Hansson B G, Dillner J
机构信息
Department of Virology, Karolinska Institute, Stockholm, Sweden.
出版信息
J Clin Microbiol. 1993 Feb;31(2):192-7. doi: 10.1128/jcm.31.2.192-197.1993.
Samples of cervical secretions and serum from 30 women with genital condylomas and 30 age-matched controls were tested for the presence of immunoglobulin A (IgA) and IgG antibodies against a panel of papillomavirus-derived antigens. The same cervical samples were also analyzed for presence of human papillomavirus (HPV) DNA by Southern blotting and polymerase chain reaction. By Southern blotting HPV DNA was detected in 8 of 30 patients with condylomas and 2 of 30 controls, and by the polymerase chain reaction HPV DNA was detected in 14 of 30 patients with condylomas and 5 of 30 controls. A total of 18 of 29 patients with condylomas and 8 of 28 controls had IgA antibodies in cervical secretions to an E2 synthetic peptide, and 17 of 29 patients with condylomas and 5 of 28 controls had local IgA antibodies to an E7 peptide (P < 0.025 and P < 0.005, respectively). The results suggest that measurement of local antibody production against selected HPV antigens may be useful in the study of HPV immunology and, possibly, for the diagnosis of HPV infection.
对30名患有生殖器湿疣的女性以及30名年龄匹配的对照者的宫颈分泌物和血清样本进行检测，以确定是否存在针对一组乳头瘤病毒衍生抗原的免疫球蛋白A（IgA）和IgG抗体。同时，通过Southern印迹法和聚合酶链反应对相同的宫颈样本进行人乳头瘤病毒（HPV）DNA检测。通过Southern印迹法，在30名湿疣患者中有8例检测到HPV DNA，30名对照者中有2例检测到；通过聚合酶链反应，30名湿疣患者中有14例检测到HPV DNA，30名对照者中有5例检测到。在29名湿疣患者中有18例以及28名对照者中有8例宫颈分泌物中存在针对E2合成肽的IgA抗体，在29名湿疣患者中有17例以及28名对照者中有5例存在针对E7肽的局部IgA抗体（P分别<0.025和P<0.005）。结果表明，检测针对特定HPV抗原的局部抗体产生可能有助于HPV免疫学研究，也可能有助于HPV感染的诊断。
Zotero 插件