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DNA复制在β-珠蛋白基因激活中的作用。

Role for DNA replication in beta-globin gene activation.

作者信息

Enver T, Brewer A C, Patient R K

机构信息

Department of Biophysics, Cell and Molecular Biology, King's College London, United Kingdom.

出版信息

Mol Cell Biol. 1988 Mar;8(3):1301-8. doi: 10.1128/mcb.8.3.1301-1308.1988.

DOI:10.1128/mcb.8.3.1301-1308.1988
PMID:2835669
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC363276/
Abstract

Transcriptional activation of the Xenopus laevis beta-globin gene requires the synergistic action of the simian virus 40 enhancer and DNA replication in DEAE-dextran-mediated HeLa cell transfections. Replication does not act through covalent modification of the template, since its requirement was not obviated by the prior replication of the transfected DNA in eucaryotic cells. Transfection of DNA over a 100-fold range demonstrates that replication does not contribute to gene activation simply increasing template copy number. Furthermore, in cotransfections of replicating and nonreplicating constructs, only replicating templates were transcribed. Replication is not simply a requirement of chromatin assembly, since even unreplicated templates generated nucleosomal ladders. Stimulation of beta-globin transcription by DNA replication, though less marked, was also observed in calcium phosphate transfections. We interpret these results as revealing a dynamic role for replication in gene activation.

摘要

在DEAE-葡聚糖介导的HeLa细胞转染中，非洲爪蟾β-珠蛋白基因的转录激活需要猿猴病毒40增强子和DNA复制的协同作用。复制并非通过模板的共价修饰起作用，因为在真核细胞中转染的DNA预先复制并不能消除对复制的需求。在超过100倍范围的DNA转染实验表明，复制并非仅仅通过增加模板拷贝数来促进基因激活。此外，在复制型和非复制型构建体的共转染实验中，只有复制型模板被转录。复制并非染色质组装的简单需求，因为即使未复制的模板也能产生核小体梯带。在磷酸钙转染实验中也观察到，尽管不太明显，但DNA复制对β-珠蛋白转录有刺激作用。我们将这些结果解释为揭示了复制在基因激活中的动态作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9efc/363276/504aaac26293/molcellb00063-0306-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9efc/363276/4b2695526bef/molcellb00063-0302-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9efc/363276/bcc126fde43c/molcellb00063-0303-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9efc/363276/2925280a4e21/molcellb00063-0304-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9efc/363276/9d8bf73afa46/molcellb00063-0304-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9efc/363276/f4d921b66ea7/molcellb00063-0305-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9efc/363276/7646792cfa0a/molcellb00063-0306-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9efc/363276/504aaac26293/molcellb00063-0306-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9efc/363276/4b2695526bef/molcellb00063-0302-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9efc/363276/bcc126fde43c/molcellb00063-0303-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9efc/363276/2925280a4e21/molcellb00063-0304-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9efc/363276/9d8bf73afa46/molcellb00063-0304-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9efc/363276/f4d921b66ea7/molcellb00063-0305-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9efc/363276/7646792cfa0a/molcellb00063-0306-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9efc/363276/504aaac26293/molcellb00063-0306-b.jpg

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A STOCHASTIC MODEL OF STEM CELL PROLIFERATION, BASED ON THE GROWTH OF SPLEEN COLONY-FORMING CELLS.基于脾集落形成细胞生长的干细胞增殖随机模型
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