Suriyaprom Kanjana, Tungtrongchitr Rungsunn, Namjuntra Pisit
Faculty of Medical Technology, Rangsit University, Paholyothin Road Pathumthani, Thailand.
Department of Tropical Nutrition & Food Science, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
J Med Biochem. 2015 Apr;34(2):170-178. doi: 10.2478/jomb-2014-0034. Epub 2015 Mar 3.
Metabolic syndrome (MS) is a clinical constellation comprising risk factors associated with developing cardiovascular disease and type 2 diabetes. Resistin has been suggested as a linkage between obesity, inflammation and type 2 diabetes. This study aimed to investigate resistin concentrations and hematological-biochemical parameters in MS subjects and controls, and to determine whether two resistin gene ( polymorphisms (-420C>G & +299G>A) are linked to resistin levels and MS among Thais.
This case-control study was performed with 322 Thai volunteers: 160 MS subjects and 162 controls. Anthropometric parameters and hematological-biochemical variables were determined. The -420C>G (rs1862513) and +299G>A (rs3745367) polymorphisms were genotyped by PCR-RFLP technique.
The resistin levels of the MS group were significantly higher than those of the control group. Resistin levels were positively correlated with anthropometric parameters and WBC count in the MS group. According to -420C>G polymorphism, MS subjects with the G allele (CG/GG) (3.9 μg/L) had significantly higher resistin concentrations than in subjects with the CC genotype (2.4 μg/L); with regard to +299G>A polymorphism, carriers with the A allele (GA/AA) (3.8 μg/L) had significantly higher resistin levels than subjects with the GG genotype (2.7 μg/L), after adjusting for potential covariates. However, the -420C>G and +299G>A polymorphisms were not found to be associated with MS, hematological-biochemical parameters and anthropometric variables.
These findings suggest resistin levels are linked with MS and the -420C>G and +299G>A polymorphisms have impacted the circulating resistin concentrations. However, these two polymorphisms probably do not influence susceptibility to MS among Thais.
代谢综合征（MS）是一组临床症候群，包含与心血管疾病和2型糖尿病发生相关的危险因素。抵抗素被认为是肥胖、炎症和2型糖尿病之间的一个联系因素。本研究旨在调查MS患者和对照组的抵抗素浓度及血液生化参数，并确定两种抵抗素基因多态性（-420C>G和+299G>A）是否与泰国人中的抵抗素水平及MS相关。
本病例对照研究纳入了322名泰国志愿者：160名MS患者和162名对照。测定了人体测量参数和血液生化变量。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术对-420C>G（rs1862513）和+299G>A（rs3745367）多态性进行基因分型。
MS组的抵抗素水平显著高于对照组。MS组中抵抗素水平与人体测量参数及白细胞计数呈正相关。根据-420C>G多态性，携带G等位基因（CG/GG）的MS患者（3.9μg/L）的抵抗素浓度显著高于CC基因型患者（2.4μg/L）；对于+299G>A多态性，在调整潜在协变量后，携带A等位基因（GA/AA）的携带者（3.8μg/L）的抵抗素水平显著高于GG基因型患者（2.7μg/L）。然而，未发现-420C>G和+299G>A多态性与MS、血液生化参数及人体测量变量相关。
这些发现表明抵抗素水平与MS相关，且-420C>G和+299G>A多态性影响了循环抵抗素浓度。然而，这两种多态性可能不会影响泰国人对MS的易感性。
