Thammakun Theerawut, Laohasiriwong Wongsa, Kraiklang Ratthaphol, Saengprajak Nittaya
Faculty, Department of Public Health, Khon Kaen University and Research and Training Center for Enhancing Quality of Life of Working Age People, Khon Kaen University , Khon Kaen, Thailand .
Associate Professor, Department of Public Health, Khon Kaen University and Board Committee of Research and Training Centre for Enhancing Quality of Life of Working Age People (REQW), Khon Kaen University , Khon Kaen, Thailand .
J Clin Diagn Res. 2017 Feb;11(2):BC15-BC20. doi: 10.7860/JCDR/2017/25072.9390. Epub 2017 Feb 1.
Resistin gene (RETN) polymorphisms in humans may have a role in the pathogenesis of Type 2 Diabetes Mellitus (T2DM) and insulin resistance. There is still lack of evidence on association between +62 G>A polymorphism in the RETN and T2DM among Thais.
To determine the effect of polymorphisms at +62 G>A of RETN on Thai T2DM.
This matched case control study was conducted with a total of 360 samples from all regions of Thailand (180 Thai new T2DM cases and 180 non-T2DM Thais for control) were enrolled. The RETN +62G>A polymorphism were detected using the Polymerase Chain Reaction (PCR) method. Conditional logistic regression was performed to test the association between +62 G>A polymorphism and T2DM.
Among 360 samples that were enrolled, only 350 samples completed molecular analysis. It was found that GA+AA genotype frequencies in T2DM cases was higher than control by 16% (95% CI: 6.0%, 27.0%, p-value=0.002). After adjustments for possible confounders, multivariable analyses by conditional logistic regression showed that the RETN+62 G>A polymorphism was statistically associated with Thai T2DM (OR =1.84, 95% CI: 1.03, 3.31, p-value=0.04). Other factors such as; low educational attainment (OR=3.87, 95%CI: 1.60, 9.36), hypertension (OR=3.07, 95%CI: 1.56, 6.04), had both obese father and mother (OR=1.94, 95%CI: 1.06, 3.56) and triglyceride≥150 (OR=2.18, 95% CI: 1.18, 4.02) were statistically associated with Thai T2DM (p-value<0.05). While regular consumption of glutinous rice was found to be a protective factor (OR=0.29, 95%CI: 0.13, 0.64).
These findings suggest that RETN polymorphism at position +62 G>A may increase the susceptibility to T2DM in Thais.
人类抵抗素基因（RETN）多态性可能在2型糖尿病（T2DM）发病机制及胰岛素抵抗中发挥作用。泰国人中，RETN基因+62 G>A多态性与T2DM之间的关联仍缺乏证据。
确定RETN基因+62 G>A多态性对泰国T2DM的影响。
开展此项匹配病例对照研究，共纳入来自泰国各地的360份样本（180例泰国新发T2DM病例及180例非T2DM泰国人作为对照）。采用聚合酶链反应（PCR）方法检测RETN基因+62G>A多态性。进行条件逻辑回归以检验+62 G>A多态性与T2DM之间的关联。
在纳入的360份样本中，仅350份样本完成分子分析。发现T2DM病例中GA+AA基因型频率比对照组高16%（95%置信区间：6.0%，27.0%，p值=0.002）。在对可能的混杂因素进行调整后，通过条件逻辑回归进行的多变量分析显示，RETN基因+62 G>A多态性与泰国T2DM存在统计学关联（比值比=1.84，95%置信区间：1.03，3.31，p值=0.04）。其他因素如：低教育程度（比值比=3.87，95%置信区间：1.60，9.36）、高血压（比值比=3.07，95%置信区间：1.56，6.04）、父母均肥胖（比值比=1.94，95%置信区间：1.06，3.56）及甘油三酯≥150（比值比=2.18，95%置信区间：1.18，4.02）与泰国T2DM均存在统计学关联（p值<0.05）。而经常食用糯米被发现是一个保护因素（比值比=0.29，95%置信区间：0.13，0.64）。
这些发现表明，RETN基因+62 G>A位点的多态性可能增加泰国人患T2DM的易感性。
