Wu Fang, Liu Yizhi, Li Jian, Hou Lei, Lei Fuxi, Huang Shangke, Feng Lu, Zhao Xinhan
Department of Medical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
Department of Medical Oncology, Shaanxi Provincial Tumor Hospital, Xi'an, Shaanxi 710061, P.R. China.
Oncol Lett. 2017 Feb;13(2):579-586. doi: 10.3892/ol.2016.5470. Epub 2016 Dec 7.
Gene therapy is one of the most promising potential therapeutic strategies for many types of cancer. Cell apoptosis is an active, programmed physiological process of the body, and its disruption has been closely associated with the occurrence of tumor development. Apoptin is known to induce tumor cell apoptosis. In the present study, the MCF-7 breast cancer cell line was transfected with a human serum albumin (HSA) and apoptin expressing plasmid [HSA-polyethylenimine (PEI)-pcDNA-Apoptin]. Reverse transcription-quantitative polymerase chain reaction and western blotting were performed to detect the expression of apoptin in the transfected MCF-7 cells, while MTT assays and flow cytometry were conducted to detect cell viability and apoptosis. Furthermore, hematoxylin and eosin staining was used to observe the morphology of xenografts from mice injected with MCF-7 cells. It was demonstrated that the HSA-PEI-pcDNA-Apoptin expression plasmid resulted in the upregulation of apoptin in MCF-7 cells, and efficiently suppressed breast tumor growth . These findings indicated that the use of HSA as an apoptin expression vector has potential therapeutic benefits for cancer and confirms the requirement for the further evaluation of apoptin in clinical trials.
基因治疗是多种癌症最具前景的潜在治疗策略之一。细胞凋亡是机体一个活跃的、程序性的生理过程，其破坏与肿瘤发生发展密切相关。已知凋亡素可诱导肿瘤细胞凋亡。在本研究中，用携带人血清白蛋白（HSA）和凋亡素的表达质粒[HSA-聚乙烯亚胺（PEI）-pcDNA-凋亡素]转染MCF-7乳腺癌细胞系。进行逆转录-定量聚合酶链反应和蛋白质免疫印迹法检测转染后MCF-7细胞中凋亡素的表达，同时进行MTT法和流式细胞术检测细胞活力和凋亡情况。此外，采用苏木精-伊红染色观察注射MCF-7细胞的小鼠异种移植瘤的形态。结果表明，HSA-PEI-pcDNA-凋亡素表达质粒可使MCF-7细胞中凋亡素表达上调，并有效抑制乳腺肿瘤生长。这些发现表明，将HSA用作凋亡素表达载体对癌症具有潜在治疗益处，并证实了在临床试验中进一步评估凋亡素的必要性。
