Shakoori Zahra, Ghanbari Hossein, Omidi Yadollah, Pashaiasl Maryam, Akbarzadeh Abolfazl, Jomeh Farsangi Zohreh, Rezayat Seyed Mahdi, Davaran Soodabeh
a Department of Medical Nanotechnology, School of Advanced Technologies in Medicine , Tehran University of Medical Sciences , Tehran , Iran.
b Faculty of Pharmacy, Research Center for Pharmaceutical Nanotechnology , Tabriz University of Medical Sciences , Tabriz , Iran.
Drug Dev Ind Pharm. 2017 Aug;43(8):1283-1291. doi: 10.1080/03639045.2017.1313859. Epub 2017 Apr 18.
Magnetic, pH and temperature-sensitive, poly(N-isopropylacrylamide) (PNIPAM)-based nanocomposites with fluorescent properties were synthesized by free radical copolymerization-cross linking of NIPAM, N,N-dimethylaminoethyl methacrylate (DMAEMA) and 4-acrylamidofluorescein (AFA). The model anti-cancer drug, cisplatin (CDDP), was loaded into the resulted nanogel. For the production of CDDP-loaded nanocomposite, FeO magnetic nanoparticles (MNPs) and CDDP were loaded into the nanogel. Field-emission scanning electron microscopy (FE-SEM) indicated that the size of nanogel and CDDP-loaded nanocomposite were about 90 and 160 nm, respectively. The encapsulation efficiency of CCDP was found up to 65%. The loaded CCDP showed sustained thermal and pH-responsive drug release. A high level of drug release was observed under the conditions of low pH and high temperature. The lower critical solution temperature (LCST) of synthesized nanogel was about 40 °C. CDDP-loaded nanocomposite showed a volume phase transition from 282 to 128 nm at its LCST. Accordingly, in this study, the synthesized nanocomposite can be employed as a stimuli-responsive anti-cancer drug delivery system and the pH and temperature of solution have the potential to monitor the drug release.
通过N-异丙基丙烯酰胺（NIPAM）、甲基丙烯酸N，N-二甲氨基乙酯（DMAEMA）和4-丙烯酰胺基荧光素（AFA）的自由基共聚合交联反应，合成了具有荧光特性的磁性、pH和温度敏感的聚（N-异丙基丙烯酰胺）（PNIPAM）基纳米复合材料。将模型抗癌药物顺铂（CDDP）负载到所得纳米凝胶中。为了制备负载CDDP的纳米复合材料，将FeO磁性纳米颗粒（MNPs）和CDDP负载到纳米凝胶中。场发射扫描电子显微镜（FE-SEM）表明，纳米凝胶和负载CDDP的纳米复合材料的尺寸分别约为90和160nm。发现CCDP的包封率高达65%。负载的CCDP表现出持续的热和pH响应药物释放。在低pH和高温条件下观察到高水平的药物释放。合成纳米凝胶的低临界溶液温度（LCST）约为40°C。负载CDDP的纳米复合材料在其LCST下显示出从282到128nm的体积相转变。因此，在本研究中，合成的纳米复合材料可作为刺激响应型抗癌药物递送系统，溶液的pH和温度有可能监测药物释放。
