Li Yanfen, Ma Souzhi, Guo Jianbin, Jiang Jun, Luo Kai, Yan Fuhua, Xiao Yin
a School and Hospital of Stomatology , Fujian Medical University , Fuzhou , China.
b Nanjing Stomatological Hospital , Medical School of Nanjing University , Nanjing , China.
Acta Odontol Scand. 2017 May;75(4):268-279. doi: 10.1080/00016357.2017.1292427. Epub 2017 Feb 24.
To investigate the effect of local hIL-10 gene therapy on experimental periodontitis in rats and to elucidate the mechanism underlying this effect.
Experimental periodontitis was induced in ovariectomized (OVX) rats using a silk ligature. We then injected 5 μg of hIL-10 plasmid with 5 μl of liposomes or 5 μg of vector plasmid with 5 μl of liposomes into the palatal side of the gingival mucosa of the upper left second molar once every two days. The rats were killed 48 hours after the seventh injection. The body weight; bone mineral density of the whole body, pelvis and spine; resorption of the alveolar bone; and number of cytokine-positive cells were measured to determine the effects of hIL-10 on the periodontal tissue.
hIL-10 was expressed in periodontal tissues after local gene delivery. The expressed hIL-10 protein inhibited alveolar bone resorption and downregulated IL-1β, IL-6, TNF-α, RANKL and MMP-8 in the periodontal ligament in the root furcation region.
Local hIL-10 gene transfer suppressed alveolar bone resorption in OVX rats, and this effect was probably associated with the decline in the expression of pro-inflammatory cytokines in the periodontal tissues.
探讨局部人白细胞介素-10（hIL-10）基因治疗对大鼠实验性牙周炎的影响，并阐明其作用机制。
采用丝线结扎法在去卵巢（OVX）大鼠中诱导实验性牙周炎。然后，每两天一次将5μg hIL-10质粒与5μl脂质体或5μg载体质粒与5μl脂质体注入左上第二磨牙牙龈黏膜的腭侧。在第七次注射后48小时处死大鼠。测量体重、全身、骨盆和脊柱的骨矿物质密度、牙槽骨吸收情况以及细胞因子阳性细胞数量，以确定hIL-10对牙周组织的影响。
局部基因递送后，hIL-10在牙周组织中表达。表达的hIL-10蛋白抑制了牙槽骨吸收，并下调了根分叉区域牙周韧带中白细胞介素-1β、白细胞介素-6、肿瘤坏死因子-α、核因子κB受体活化因子配体（RANKL）和基质金属蛋白酶-8（MMP-8）的表达。
局部hIL-10基因转移抑制了OVX大鼠的牙槽骨吸收，这种作用可能与牙周组织中促炎细胞因子表达的下降有关。
