Cavalla Franco, Biguetti Claudia Cristina, Melchiades Jessica Lima, Tabanez Andre Pantenuci, Azevedo Michelle de Campos Soriani, Trombone Ana Paula Favaro, Faveri Marcelo, Feres Magda, Garlet Gustavo Pompermaier
Conservative Dentistry Department, School of Dentistry University of Chile, Santiago 8380544, Chile.
Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru 17012-901, São Paulo, Brazil.
Genes (Basel). 2018 May 23;9(6):271. doi: 10.3390/genes9060271.
Chronic periodontitis is the most prevalent form of inflammatory destructive bone disease and has been affecting humans since antiquity. Evidence suggest that genetic factors can highly influence periodontitis risk, modulating disease elements such as the susceptibility to microbial colonization and the nature of subsequent host-microbe interaction. Several single-nucleotide polymorphisms (SNPs) have been associated with the occurrence of periodontitis, but the full range of genetic influence in periodontitis outcomes remains to be determined. In this context, this study comprises an analysis of possible correlation between periodontitis-related genetic variants with changes in the subgingival microbiological pattern performed in a Brazilian population ( = 167, comprising 76 chronic periodontitis patients and 91 healthy subjects). For the genetic characterization, 19 candidate SNPs were selected based on the top hits of previous large genome wide association studies (GWAS), while the subgingival microbiota was characterized for the presence and relative quantity of 40 bacterial species by DNA-DNA checkerboard. The case/control association test did not demonstrate a significant effect of the target SNPs with the disease phenotype. The polymorphism rs2521634 proved significantly associated with , , and ; rs10010758 and rs6667202 were associated with increased counts of ; and rs10043775 proved significantly associated with decreased counts of . In conclusion, we present strong evidence supporting a direct connection between the host's genetic profile, specifically rs2521634, rs10010758, rs6667202, and rs10043775 polymorphisms, and the occurrence of chronic periodontitis-associated bacteria.
慢性牙周炎是炎症性破坏性骨疾病最常见的形式，自古以来一直影响着人类。有证据表明，遗传因素可高度影响牙周炎风险，调节诸如微生物定植易感性和随后宿主 - 微生物相互作用性质等疾病因素。若干单核苷酸多态性（SNP）已与牙周炎的发生相关，但牙周炎结局中遗传影响的全貌仍有待确定。在此背景下，本研究分析了巴西人群（n = 167，包括76例慢性牙周炎患者和91名健康受试者）中牙周炎相关基因变异与龈下微生物模式变化之间的可能相关性。为进行基因特征分析，基于先前大型全基因组关联研究（GWAS）的显著结果选择了19个候选SNP，同时通过DNA - DNA杂交点阵法对龈下微生物群的40种细菌的存在和相对数量进行了特征分析。病例/对照关联测试未显示目标SNP对疾病表型有显著影响。多态性rs2521634被证明与[具体细菌名称未给出]、[具体细菌名称未给出]、[具体细菌名称未给出]和[具体细菌名称未给出]显著相关；rs10010758和rs6667202与[具体细菌名称未给出]数量增加相关；rs10043775被证明与[具体细菌名称未给出]数量减少显著相关。总之，我们提供了有力证据，支持宿主基因谱，特别是rs2521634、rs10010758、rs6667202和rs10043775多态性，与慢性牙周炎相关细菌的发生之间存在直接联系。
