Schuerch Kaspar, Woods Russell L, Lee Winston, Duncker Tobias, Delori François C, Allikmets Rando, Tsang Stephen H, Sparrow Janet R
Department of Ophthalmology, Columbia University, New York, New York, United States.
Schepens Eye Research Institute and Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, United States.
Invest Ophthalmol Vis Sci. 2017 Mar 1;58(3):1843-1855. doi: 10.1167/iovs.16-21302.
Using quantitative fundus autofluorescence (qAF), we analyzed short-wavelength autofluorescent (SW-AF) rings in RP.
Short-wavelength autofluorescent images (486 nm excitation) of 40 patients with RP (69 eyes) were acquired with a confocal scanning laser ophthalmoscope equipped with an internal fluorescent reference. Mean qAF was measured in eight preset segments (qAF8) and in region of interest (ROI)-qAF (200-700 μm) within and external to the borders of the rings at superior, temporal, and inferior sites relative to the ring. For both groups, qAF in patients with RP was compared to age-similar and race/ethnicity-matched healthy eyes at equivalent retinal locations.
In 71% of eyes of RP patients, qAF8 acquired internal to the inner border of the ring, was within the 95% confidence interval (CI) for healthy eyes, while in the remaining RP eyes qAF8 was either higher or lower than the CI. Measured external to the ring, qAF8 values were within the CI in 47% of RP eyes with the other eyes being higher or lower. In 28% of sites measured by ROI-qAF within the SW-AF ring, values were above the 95% CI of healthy controls. Region of interest-qAF measured just external to the ring was within the CI of healthy eyes in 74% of locations. The average local elevation in qAF within the ring was approximately 15%. In SD-OCT scans, photoreceptor-attributable reflectivity bands were thinned within and external to the ring.
Increased fluorophore production may be a factor in the formation of the SW-AF rings in RP.
我们使用定量眼底自发荧光（qAF）分析了视网膜色素变性（RP）中的短波自发荧光（SW-AF）环。
使用配备内部荧光参考的共焦扫描激光检眼镜获取40例RP患者（69只眼）的短波自发荧光图像（486nm激发）。在相对于环的上方、颞侧和下方部位，在环边界内和外的八个预设节段（qAF8）以及感兴趣区域（ROI）-qAF（200-700μm）中测量平均qAF。对于两组，将RP患者的qAF与年龄相似且种族/民族匹配的健康眼在等效视网膜位置进行比较。
在RP患者71%的眼中，在环内边界内部获取的qAF8在健康眼的95%置信区间（CI）内，而在其余的RP眼中，qAF8要么高于要么低于CI。在环外测量时，47%的RP眼的qAF8值在CI内，其他眼则更高或更低。在SW-AF环内通过ROI-qAF测量的28%的部位，值高于健康对照的95%CI。在环外紧邻处测量的感兴趣区域-qAF在74%的位置在健康眼的CI内。环内qAF的平均局部升高约为15%。在SD-OCT扫描中，环内和环外可归因于光感受器的反射带变薄。
荧光团产生增加可能是RP中SW-AF环形成的一个因素。
