Wake Forest Institute for Regenerative Medicine, Fetal Research and Therapy Program Wake Forest School of Medicine, Winston-Salem, NC 27157, USA.
Wake Forest Institute for Regenerative Medicine, Fetal Research and Therapy Program Wake Forest School of Medicine, Winston-Salem, NC 27157, USA.
Semin Cell Dev Biol. 2021 Apr;112:1-7. doi: 10.1016/j.semcdb.2020.06.002. Epub 2020 Jun 18.
The ability to study the behavior of cells, proteins, and cell-cell or cell-protein interactions under dynamic forces such as shear stress under fluid flow, provides a more accurate understanding of the physiopathology of hemostasis. This review touches upon the traditional methods for studying blood coagulation and platelet aggregation and provides an overview on cellular and protein response to shear stress. We also elaborate on the biological aspects of how cells recognize mechanical forces and convert them into biochemical signals that can drive various signaling pathways. We give a detailed description of the various types of microfluidic devices that are employed to study the complex processes of platelet aggregation and blood coagulation under flow conditions as well as to investigate endothelial shear-response. We also highlight works mimicking artificial vessels as platforms to study the mechanisms of coagulation, and finish our review by describing anticipated clinical uses of microfluidics devices and their standardization.
在血流剪切力等动态力下研究细胞、蛋白质以及细胞-细胞或细胞-蛋白质相互作用的行为，能够更准确地理解止血的病理生理学。本文回顾了传统的血液凝固和血小板聚集研究方法，并概述了细胞和蛋白质对剪切力的反应。我们还详细阐述了细胞如何识别机械力并将其转化为生化信号，从而驱动各种信号通路的生物学方面。我们详细描述了各种类型的微流控装置，这些装置用于研究流动条件下血小板聚集和血液凝固的复杂过程，以及研究内皮剪切反应。我们还强调了模拟人工血管的工作，将其作为研究凝血机制的平台，并通过描述微流控装置的预期临床用途及其标准化来完成我们的综述。
