Stirtzinger T, Valli V E, Miller J M
Department of Pathology, Ontario Veterinary College, University of Guelph.
Can J Vet Res. 1988 Apr;52(2):222-8.
Twenty-four, six month old lambs were assembled into four groups of five animals each and one group of four animals. All groups were inoculated with lymphocytes from a single donor lamb infected with bovine leukemia virus. The inoculum varied from 250 to 250,000 lymphocytes, in tenfold increments. Animals were exposed by intradermal injection in the neck region immediately anterior to the left shoulder joint. All groups were monitored at 0, 3, 7 and 12 weeks after inoculation using the following procedures: a. Syncytia induction assay for detection of bovine leukemia virus in peripheral blood lymphocytes. b. Agar gel immunodiffusion against the gp51 antigen of bovine leukemia virus for the detection of antibovine leukemia virus gp51 antibody. c. Lymphocyte stimulation test for the assessment of cell-mediated immunity using mitogen, nonfractionated bovine leukemia virus antigen, and partially purified bovine lymphoma tumor-associated antigen for the in vitro activation of lymphocytes from bovine leukemia virus-inoculated and sham-inoculated, control animals. d. Routine hematological techniques for the assessment of total leukocyte and lymphocyte counts. The median infectious dose for lymphocytes from the single bovine leukemia virus-infected donor used in this study was determined to be 2000 cells. The syncytia induction assay detected more infected individuals (13/23) at an earlier time than did the agar gel immunodiffusion assay (10/23). Using either serological or virus isolation techniques, infected animals were first detected at three weeks postinoculation in the group receiving the high-dose inoculum and at seven weeks postinoculation in groups receiving low- or medium-dose inocula.(ABSTRACT TRUNCATED AT 250 WORDS)
24只6月龄的羔羊被分成四组，每组5只，另一组4只。所有组均接种来自一只感染牛白血病病毒的供体羔羊的淋巴细胞。接种物的数量从250个淋巴细胞到250,000个淋巴细胞不等，以10倍递增。通过在左肩关节前方颈部区域进行皮内注射使动物接触病毒。接种后0、3、7和12周，使用以下程序对所有组进行监测：a. 用于检测外周血淋巴细胞中牛白血病病毒的多核巨细胞诱导试验。b. 针对牛白血病病毒gp51抗原的琼脂凝胶免疫扩散试验，用于检测抗牛白血病病毒gp51抗体。c. 淋巴细胞刺激试验，使用有丝分裂原、未分级的牛白血病病毒抗原和部分纯化的牛淋巴瘤肿瘤相关抗原，对来自接种牛白血病病毒和假接种的对照动物的淋巴细胞进行体外激活，以评估细胞介导的免疫。d. 用于评估总白细胞和淋巴细胞计数的常规血液学技术。本研究中使用的来自单一感染牛白血病病毒供体的淋巴细胞的半数感染剂量被确定为2000个细胞。多核巨细胞诱导试验比琼脂凝胶免疫扩散试验更早地检测到更多感染个体（13/23比10/23）。使用血清学或病毒分离技术，在接受高剂量接种物的组中，接种后3周首次检测到感染动物，在接受低剂量或中等剂量接种物的组中，接种后7周首次检测到感染动物。（摘要截断于250字）
