Kenyon S J, Ferrer J F, McFeely R A, Graves D C
J Natl Cancer Inst. 1981 Nov;67(5):1157-63.
Newborn sheep inoculated with phytohemagglutinin (PHA)-treated short-term, cultures of lymphocytes from cattle infected with bovine leukemia virus (BLV) or with a BLV- infected long-term culture of bovine leukemic lymphocytes became persistently infected with BLV. Fifty percent or more of the sheep died with histologically confirmed lymphosarcomas. Cytogenetic studies of representative cases demonstrated that the tumors did not result from the progressive growth of neoplastic lymphocytes in the inoculum but rather from the neoplastic transformation of the recipients lymphoid cells. Neither BLV infection nor lymphosarcoma was observed in control uninoculated sheep or in sheep given injections of PHA-treated cultures of lymphocytes from BLV-free cows. The virus recovered from the tumorous sheep was indistinguishable from BLV morphologically, antigenically, and biologically, and its reverse transcriptase had the same cation preference and immunologic properties as the BLV enzyme. Persistent BLV infection and lymphosarcoma were also observed in a group of sheep inoculated neonatally with BLV-containing cell-free culture supernatants. These results extend previous observations on the high susceptibility of sheep to BLV infection and provide definitive evidence that BLV is a tumor-inducing virus.
给新生绵羊接种经植物血凝素（PHA）处理的、来自感染牛白血病病毒（BLV）的牛的短期淋巴细胞培养物，或接种来自感染BLV的牛白血病淋巴细胞的长期培养物后，这些绵羊会持续感染BLV。50%或更多的绵羊死于经组织学证实的淋巴肉瘤。对代表性病例的细胞遗传学研究表明，肿瘤并非源于接种物中肿瘤性淋巴细胞的渐进性生长，而是源于受体淋巴样细胞的肿瘤性转化。在未接种的对照绵羊或注射了来自无BLV牛的经PHA处理的淋巴细胞培养物的绵羊中，未观察到BLV感染或淋巴肉瘤。从患肿瘤绵羊中分离出的病毒在形态、抗原性和生物学特性上与BLV无法区分其逆转录酶与BLV酶具有相同的阳离子偏好和免疫特性。在一组新生时接种含BLV的无细胞培养上清液的绵羊中，也观察到了持续的BLV感染和淋巴肉瘤。这些结果扩展了先前关于绵羊对BLV感染高度易感性的观察，并提供了确凿证据表明BLV是一种致瘤病毒。
