Immune Regulates CD11c Cells by Interacting with the Macrophage Gal/GalNAc Lectin.

Fasciolosis, caused by and , is a trematode zoonosis of interest in public health and livestock production. Like other helminths, modulates the host immune response by inducing potent polarized Th2 and regulatory T cell immune responses and by downregulating the production of Th1 cytokines. In this work, we show that glycans increase Th2 immune responses by immunomodulating TLR-induced maturation and function of dendritic cells (DCs). This process was mediated by the macrophage Gal/GalNAc lectin (MGL) expressed on DCs, which recognizes the Tn antigen (GalNAc-Ser/Thr) on parasite components. More interestingly, we identified MGL-expressing CD11c cells in infected animals and showed that these cells are recruited both to the peritoneum and the liver upon infection. These cells express the regulatory cytokines IL-10, TNFα and TGFβ and a variety of regulatory markers. Furthermore, MGL CD11c cells expand parasite-specific Th2/regulatory cells and suppress Th1 polarization. The results presented here suggest a potential role of MGL in the immunomodulation of DCs induced by and contribute to a better understanding of the molecular and immunoregulatory mechanisms induced by this parasite.