Favre Laurie, Ortalo-Magné Annick, Greff Stéphane, Pérez Thierry, Thomas Olivier P, Martin Jean-Charles, Culioli Gérald
Université de Toulon , MAPIEM, EA 4323, La Garde Cedex 83130, France.
CNRS, Aix Marseille Univ , IRD, Avignon Univ. Institut Méditerranéen de Biodiversité et d'Ecologie marine et continentale, Station marine d'Endoume, Marseille 13007, France.
J Proteome Res. 2017 May 5;16(5):1962-1975. doi: 10.1021/acs.jproteome.6b01027. Epub 2017 Apr 12.
Most marine bacteria can form biofilms, and they are the main components of biofilms observed on marine surfaces. Biofilms constitute a widespread life strategy, as growing in such structures offers many important biological benefits. The molecular compounds expressed in biofilms and, more generally, the metabolomes of marine bacteria remain poorly studied. In this context, a nontargeted LC-MS metabolomics approach of marine biofilm-forming bacterial strains was developed. Four marine bacteria, Persicivirga (Nonlabens) mediterranea TC4 and TC7, Pseudoalteromonas lipolytica TC8, and Shewanella sp. TC11, were used as model organisms. The main objective was to search for some strain-specific bacterial metabolites and to determine how culture parameters (culture medium, growth phase, and mode of culture) may affect the cellular metabolism of each strain and thus the global interstrain metabolic discrimination. LC-MS profiling and statistical partial least-squares discriminant analyses showed that the four strains could be differentiated at the species level whatever the medium, the growth phase, or the mode of culture (planktonic vs biofilm). A MS/MS molecular network was subsequently built and allowed the identification of putative bacterial biomarkers. TC8 was discriminated by a series of ornithine lipids, while the P. mediterranea strains produced hydroxylated ornithine and glycine lipids. Among the P. mediterranea strains, TC7 extracts were distinguished by the occurrence of diamine derivatives, such as putrescine amides.
大多数海洋细菌都能形成生物膜，它们是在海洋表面观察到的生物膜的主要组成部分。生物膜构成了一种广泛存在的生存策略，因为在这样的结构中生长能带来许多重要的生物学益处。生物膜中表达的分子化合物，以及更一般地说，海洋细菌的代谢组，仍然研究不足。在此背景下，开发了一种针对海洋生物膜形成细菌菌株的非靶向液相色谱 - 质谱代谢组学方法。四种海洋细菌，地中海假丝菌（非拉本氏菌）TC4和TC7、解脂假交替单胞菌TC8以及希瓦氏菌属TC11，被用作模式生物。主要目的是寻找一些菌株特异性的细菌代谢物，并确定培养参数（培养基、生长阶段和培养模式）如何影响每个菌株的细胞代谢，进而影响菌株间的整体代谢差异。液相色谱 - 质谱分析和统计偏最小二乘判别分析表明，无论培养基、生长阶段或培养模式（浮游与生物膜）如何，这四种菌株在物种水平上都可以区分。随后构建了一个串联质谱分子网络，并鉴定出了假定的细菌生物标志物。TC8可通过一系列鸟氨酸脂质来区分，而地中海假丝菌菌株产生羟基化鸟氨酸和甘氨酸脂质。在地中海假丝菌菌株中，TC7提取物的特征是存在二胺衍生物，如腐胺酰胺。
