Shahid Izzah, Han Jun, Hanook Sharoon, Borchers Christoph H, El Enshasy Hesham Ali, Mehnaz Samina
Department of Biotechnology, Faculty of Science and Technology, University of Central Punjab, Lahore, Pakistan.
University of Victoria-Genome BC Proteomics Center, University of Victoria, Victoria, BC V8Z 7X8 Canada.
3 Biotech. 2023 Jun;13(6):182. doi: 10.1007/s13205-023-03607-x. Epub 2023 May 12.
The recent advances in omics and computational analysis have enabled the capacity to identify the exclusive strain-specific metabolites and novel biosynthetic gene clusters. This study analyzed eight strains of including GS1, GS3, GS4, GS6, GS7, FS2, ARS38, PBSt2, one strain of RP4, one strain of (At1RP4), and one strain of (RS1) for the production of rhamnolipids, quorum-sensing signals, and osmolytes. Seven rhamnolipid derivatives were variably detected in fluorescent pseudomonads. These rhamnolipids included Rha-C-C, Rha-Rha-C-C, Rha-C-Cdb, Rha-C-C, Rha-Rha-C-C Rha-C-C, and Rha-Rha-C-Cdb. spp. also showed the variable production of osmoprotectants including N-acetyl glutaminyl glutamine amide (NAGGN), betaine, ectoine, and trehalose. Betaine and ectoine were produced by all pseudomonads, however, NAGGN and trehalose were observed by five and three strains, respectively. Four strains including (RP4), (At1RP4), (RS1), and (PBSt2) were exposed to 1- 4% NaCl concentrations and evaluated for the changes in phenazine production profile which were negligible. AntiSMASH 5.0 platform showed 50 biosynthetic gene clusters in PB-St2, of which 23 (45%) were classified as putative gene clusters with ClusterFinder algorithm, five (10%) were classified as non-ribosomal peptides synthetases (NRPS), five (10%) as saccharides, and four (8%) were classified as putative fatty acids. The genomic attributes and comprehensive insights into the metabolomic profile of these spp. strains showcase their phytostimulatory, phyto-protective, and osmoprotective effects of diverse crops grown in normal and saline soils.
The online version contains supplementary material available at 10.1007/s13205-023-03607-x.
组学和计算分析的最新进展使得能够识别独特的菌株特异性代谢物和新的生物合成基因簇。本研究分析了包括GS1、GS3、GS4、GS6、GS7、FS2、ARS38、PBSt2在内的8株荧光假单胞菌、1株恶臭假单胞菌RP4、1株嗜麦芽窄食单胞菌（At1RP4）和1株斯氏假单胞菌（RS1）产生鼠李糖脂、群体感应信号和渗透保护剂的情况。在荧光假单胞菌中可变地检测到7种鼠李糖脂衍生物。这些鼠李糖脂包括鼠李糖-C-C、鼠李糖-鼠李糖-C-C、鼠李糖-C-Cdb、鼠李糖-C-C、鼠李糖-鼠李糖-C-C、鼠李糖-C-C和鼠李糖-鼠李糖-C-Cdb。假单胞菌属还显示出包括N-乙酰谷氨酰胺基谷氨酰胺酰胺（NAGGN）、甜菜碱、四氢嘧啶和海藻糖在内的渗透保护剂的可变产生。所有假单胞菌都产生甜菜碱和四氢嘧啶，然而，分别有5株和3株观察到NAGGN和海藻糖。包括恶臭假单胞菌（RP4）、嗜麦芽窄食单胞菌（At1RP4）、斯氏假单胞菌（RS1）和荧光假单胞菌（PBSt2）在内的4株菌株暴露于1%-4%的NaCl浓度下，并评估吩嗪产生谱的变化，其变化可忽略不计。AntiSMASH 5.0平台显示PB-St2中有50个生物合成基因簇，其中23个（45%）被ClusterFinder算法分类为推定基因簇，5个（10%）被分类为非核糖体肽合成酶（NRPS），五个（10%）为糖类，四个（8%）被分类为推定脂肪酸。对这些假单胞菌属菌株的基因组特征和代谢组学图谱的全面洞察展示了它们对正常和盐渍土壤中生长的多种作物的植物刺激、植物保护和渗透保护作用。
在线版本包含可在10.1007/s13205-023-03607-x获取的补充材料。
