Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang, Marine Science School, Ningbo University, Ningbo 315211, Zhejiang, China.
Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang, Marine Science School, Ningbo University, Ningbo 315211, Zhejiang, China.
Carbohydr Polym. 2017 Jun 1;165:344-350. doi: 10.1016/j.carbpol.2017.02.031. Epub 2017 Feb 14.
Selenium is an essential trace element for human with the antitumor properties. In the present study, the peptidoglycan (PG) derived from Lactobacillus acidophilus was modified by selenylation with the HNO-NaSeO method. Reaction temperature, reaction duration and the selenide content were optimized according to orthogonal design of three-factors. In addition, the molecular structure of selenizing peptidoglycan (Se-PG) was determined by infrared spectroscopy analysis. Furthermore, the antitumor activity of Se-PG was also investigated in HT-29 cells. The results showed that Se-PG exerted a greater antitumor activity than non-modified PG in a dose-dependent manner. These findings indicated that selenylation modification can enhance the antitumor activity of PG, and Se-PG could achieve its potential in antitumor activity.
硒是人体必需的微量元素，具有抗肿瘤特性。本研究采用 HNO-NaSeO 法对来源于嗜酸乳杆菌的肽聚糖（PG）进行硒化修饰，根据三因素正交设计优化了反应温度、反应时间和硒化物含量。此外，还通过红外光谱分析确定了硒化肽聚糖（Se-PG）的分子结构。进一步研究了 Se-PG 在 HT-29 细胞中的抗肿瘤活性。结果表明，Se-PG 以剂量依赖的方式表现出比未修饰 PG 更强的抗肿瘤活性。这些发现表明硒化修饰可以增强 PG 的抗肿瘤活性，Se-PG 可能在抗肿瘤活性方面具有潜力。
