Department of Food Science and Nutrition, Jinling College, Nanjing Normal University, Nanjing 210097, Jiangsu, China.
Department of Food Science and Nutrition, Jinling College, Nanjing Normal University, Nanjing 210097, Jiangsu, China; Food Science and Technology Department, Marine Science School, Ningbo University, Ningbo 315211, Zhejiang, China.
Carbohydr Polym. 2015 Sep 5;128:130-7. doi: 10.1016/j.carbpol.2015.04.026. Epub 2015 Apr 22.
Lactobacillus species are potential probiotic bacteria for humans because of their capacity to improve certain biological functions in the host's immune system. In this study, we focused on three peptidoglycans (PGNs) derived from different Lactobacillus strains and investigated each PGN's anti-inflammatory capacity. Each PGN was analyzed using HPLC, MALDI-TOF/TOF MS and FTIR. All three PGNs displayed a β-1,4-linked N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) structure with some modifications in the polypeptides at the end of the MurNAc residue. In a new insight, we found that PGNs inhibit the release of inflammatory cytokines in LPS-induced RAW 264.7 cells; a capacity that may be related to the TLR-4 pathway. The goal for exploring PGN diversity in Lactobacillus strains is to better understand the potential use of Lactobacillus PGNs in food and pharmaceutical applications.
乳酸菌属的某些种被认为是人类潜在的益生菌，因为它们能够改善宿主免疫系统的某些生物学功能。在这项研究中，我们专注于三种源自不同乳酸菌株的肽聚糖（PGN），并研究了每种 PGN 的抗炎能力。采用 HPLC、MALDI-TOF/TOF MS 和 FTIR 对每种 PGN 进行了分析。三种 PGN 均显示出 β-1,4 连接的 N-乙酰基胞壁酸（MurNAc）和 N-乙酰氨基葡萄糖（GlcNAc）结构，在 MurNAc 残基末端的多肽中有一些修饰。在一个新的见解中，我们发现 PGN 抑制 LPS 诱导的 RAW 264.7 细胞中炎症细胞因子的释放；这种能力可能与 TLR-4 途径有关。探索乳酸菌株中 PGN 多样性的目的是为了更好地理解乳酸菌 PGN 在食品和药物应用中的潜在用途。
