Food Science & Nutrition Department, Nanjing Normal University, Nanjing 210097, PR China.
Carbohydr Polym. 2013 Jul 25;96(2):466-73. doi: 10.1016/j.carbpol.2013.04.028. Epub 2013 Apr 18.
Probiotic bacteria, especially the Lactobacillus species, can potentially play a significant role in the antiviral and antimicrobial activity of a host's immune system. Lactobacillus acidophilus, an intestinal bacterium, is involved in the intestinal epithelial cell response when a pathogen adheres to a cell. In this study, the structure of peptidoglycan (PGN) isolated from L. acidophilus was determined with the help of HPLC, NMR, FT-IR and MALDI-TOF/TOF MS. The molecular mass of PGN is 875.260 Da. The anti-inflammatory capacity of PGN was evaluated in model RAW 264.7 cells. Epifluorescence microscopy images and western blot analysis provided compelling evidence of PGN's anti-inflammatory capacity on LPS-induced macrophages. A significant decrease in inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) levels when PGN was added up to 200 μg/ml. These data provide new insight into the cellular and molecular mechanisms by which probiotic bacteria can contribute to maintaining good health.
益生菌，特别是乳酸菌属，在宿主免疫系统的抗病毒和抗菌活性中可能发挥重要作用。嗜酸乳杆菌是一种肠道细菌，当病原体附着在细胞上时，它会参与肠道上皮细胞的反应。在这项研究中，借助 HPLC、NMR、FT-IR 和 MALDI-TOF/TOF MS 确定了从嗜酸乳杆菌中分离出的肽聚糖 (PGN) 的结构。PGN 的分子量为 875.260 Da。在 RAW 264.7 细胞模型中评估了 PGN 的抗炎能力。荧光显微镜图像和 Western blot 分析提供了令人信服的证据，证明 PGN 对 LPS 诱导的巨噬细胞具有抗炎作用。当添加高达 200 μg/ml 的 PGN 时，诱导型一氧化氮合酶 (iNOS) 和环氧化酶-2 (COX-2) 的水平显著降低。这些数据为益生菌如何有助于维持健康提供了新的见解，揭示了其在细胞和分子水平上的作用机制。
