College of Chemical Engineering and Materials Science, Tianjin University of Science & Technology, No. 29, 13th Avenue, TEDA, Tianjin 300457, China.
Key Laboratory of Industrial Fermentation Microbiology (Ministry of Education), Tianjin University of Science & Technology, Tianjin 300457, China.
Carbohydr Polym. 2017 Jun 1;165:455-461. doi: 10.1016/j.carbpol.2017.02.085. Epub 2017 Feb 22.
Herein, carboxymethyl chitosan (CMCh) supramolecular hydrogels cross-linked by metal ions (Ag, Cu and Zn) are reported. The hydrogels were formed within a few seconds by simple mixing of CMCh solution with metallic salt solutions at an appropriate pH. The prepared hydrogels were characterized by using FT-IR, XRD, SEM and rotational rheometery. FT-IR measurements suggested that the facile complexation of metal ions with carboxylic, amino and hydroxyl groups of CMCh chains promoted the rapid hydrogelation. SEM images revealed a cross-linked structure of hydrogels, while microstructural openings were observed by cross-sectional studies of the freeze-dried hydrogels. Moreover, the hydrogels showed a remarkable moldability to form free standing objects. The antibacterial activities of the hydrogels were also studied against Escherichia coli and Staphylococcus aureus by agar well diffusion method. The results demonstrated an excellent antibacterial activity of the supramolecular hydrogels. Therefore, the developed CMCh supramolecular hydrogels might be used effectively in biomedical field.
本文报道了通过金属离子（Ag、Cu 和 Zn）交联的羧甲基壳聚糖（CMCh）超分子水凝胶。将 CMCh 溶液与金属盐溶液在适当 pH 值下简单混合几秒钟即可形成水凝胶。通过傅里叶变换红外光谱（FT-IR）、X 射线衍射（XRD）、扫描电子显微镜（SEM）和旋转流变仪对制备的水凝胶进行了表征。FT-IR 测量表明，金属离子与 CMCh 链上的羧基、氨基和羟基的简单配合促进了快速水凝胶化。SEM 图像显示了水凝胶的交联结构，而通过对冻干水凝胶的横截面研究观察到微观结构开口。此外，水凝胶表现出出色的可模塑性，可形成独立的物体。通过琼脂孔扩散法研究了水凝胶对大肠杆菌和金黄色葡萄球菌的抗菌活性。结果表明，超分子水凝胶具有优异的抗菌活性。因此，开发的 CMCh 超分子水凝胶可能在生物医学领域得到有效应用。
