Continuous production of antibacterial carboxymethyl chitosan-zinc supramolecular hydrogel fiber using a double-syringe injection device.

Large-scale production of an antibacterial hydrogel is of critical importance for its practical application in biomedical field. In this regard, herein we report on the construction of a double-syringe injection device by using all the commercial parts and its use for continuous production of carboxymethyl chitosan-zinc (CMCh-Zn) supramolecular hydrogel fiber. The resultant CMCh-Zn hydrogel fibers exhibit good stretchability and knittability. The Zn loading into the hydrogel fibers can be easily controlled by adjusting the concentration of Zn solution. Scanning electron microscope measurements indicate that the CMCh-Zn hydrogel fibers have a relatively smooth and thin skin layer, as well as, a 3-dimensional interconnected microporous interior architecture. Antibacterial activities of the CMCh-Zn hydrogel fibers against both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli are also investigated. The results show that the intrinsic blue fluorescence of the as-prepared CMCh-Zn hydrogel fibers can be employed as optical indicator of their antibacterial effectiveness.