College of Chemical Engineering and Materials Science, Tianjin Key Laboratory of Brine Chemical Engineering and Resource Eco-utilization, Tianjin University of Science & Technology, No.29, 13th Avenue, TEDA, Tianjin 300457, China.
Key Laboratory of Industrial Fermentation Microbiology (Ministry of Education), Tianjin University of Science & Technology, No.29, 13th Avenue, TEDA, Tianjin 300457, China.
Int J Biol Macromol. 2020 Aug 1;156:252-261. doi: 10.1016/j.ijbiomac.2020.04.073. Epub 2020 Apr 11.
Large-scale production of an antibacterial hydrogel is of critical importance for its practical application in biomedical field. In this regard, herein we report on the construction of a double-syringe injection device by using all the commercial parts and its use for continuous production of carboxymethyl chitosan-zinc (CMCh-Zn) supramolecular hydrogel fiber. The resultant CMCh-Zn hydrogel fibers exhibit good stretchability and knittability. The Zn loading into the hydrogel fibers can be easily controlled by adjusting the concentration of Zn solution. Scanning electron microscope measurements indicate that the CMCh-Zn hydrogel fibers have a relatively smooth and thin skin layer, as well as, a 3-dimensional interconnected microporous interior architecture. Antibacterial activities of the CMCh-Zn hydrogel fibers against both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli are also investigated. The results show that the intrinsic blue fluorescence of the as-prepared CMCh-Zn hydrogel fibers can be employed as optical indicator of their antibacterial effectiveness.
大规模生产抗菌水凝胶对于其在生物医学领域的实际应用至关重要。在这方面，本文报告了使用所有商业部件构建双注射器注射装置，并将其用于连续生产羧甲基壳聚糖-锌（CMCh-Zn）超分子水凝胶纤维。所得的 CMCh-Zn 水凝胶纤维具有良好的拉伸性和可编织性。通过调整 Zn 溶液的浓度，可以轻松控制水凝胶纤维中的 Zn 负载量。扫描电子显微镜测量表明，CMCh-Zn 水凝胶纤维具有相对光滑和薄的皮层，以及 3 维相互连接的微孔内部结构。还研究了 CMCh-Zn 水凝胶纤维对革兰氏阳性金黄色葡萄球菌和革兰氏阴性大肠杆菌的抗菌活性。结果表明，所制备的 CMCh-Zn 水凝胶纤维的固有蓝色荧光可用作其抗菌效果的光学指示剂。
