College of Chemical Engineering and Materials Science, Tianjin Key Laboratory of Brine Chemical Engineering and Resource Eco-utilization, Tianjin University of Science & Technology, No.29, 13th Avenue, TEDA, Tianjin 300457, China.
College of Chemical Engineering and Materials Science, Tianjin Key Laboratory of Brine Chemical Engineering and Resource Eco-utilization, Tianjin University of Science & Technology, No.29, 13th Avenue, TEDA, Tianjin 300457, China.
Int J Biol Macromol. 2020 Jun 1;152:50-56. doi: 10.1016/j.ijbiomac.2020.02.260. Epub 2020 Feb 24.
Recently, biopolymer-based non-traditional luminogens had attracted a great deal of interest because of their potential applications in biomedical field. Herein, we report for the first time that carboxymethyl chitosan (CMCh) can exhibit strong blue fluorescence at λ = 436.8 nm when brought in contact with zinc ion (Zn) in both solution and hydrogel states. The resultant CMCh-Zn sample exhibits a typical fluorescence lifetime of 3.68 ns and a quantum yield of 6.8%. The fluorescence behaviors of CMCh-Zn samples at different excitation wavelengths, CMCh concentrations, temperature, and pH values, are also investigated. The results clearly indicate clustering-triggered emission characteristic of the CMCh-Zn. In order to further elucidate the chemical nature of this new fluorescence system, a series of CMCh-Zn samples are characterized by using ultraviolet-visible spectrometer, Fourier-transform infrared spectrometer and X-ray diffractometer. The data suggest that the metal-ligand complexation of CMCh with Zn account for the generation of such an enhanced fluorescence.
最近，基于生物聚合物的非传统发光体因其在生物医学领域的潜在应用而引起了极大的兴趣。在此，我们首次报道了当羧甲基壳聚糖（CMCh）与锌离子（Zn）接触时，无论是在溶液还是水凝胶状态下，都能在 λ=436.8nm 处表现出强烈的蓝色荧光。所得的 CMCh-Zn 样品表现出典型的荧光寿命为 3.68ns 和量子产率为 6.8%。还研究了 CMCh-Zn 样品在不同激发波长、CMCh 浓度、温度和 pH 值下的荧光行为。结果清楚地表明了 CMCh-Zn 的聚集触发发射特性。为了进一步阐明这种新荧光体系的化学性质，用紫外-可见分光光度计、傅里叶变换红外光谱仪和 X 射线衍射仪对一系列 CMCh-Zn 样品进行了表征。数据表明，CMCh 与 Zn 的金属配体络合是产生这种增强荧光的原因。
