β-半乳糖苷酶基因（lacZ）和乳糖抑制蛋白基因（lacI）信使核糖核酸（mRNA）降解过程中核酸内切酶切割的证据。
Evidence for endonucleolytic cleavages in decay of lacZ and lacI mRNAs.
作者信息
Subbarao M N, Kennell D
机构信息
Department of Microbiology and Immunology, Washington University School of Medicine, St. Louis, Missouri 63110.
出版信息
J Bacteriol. 1988 Jun;170(6):2860-5. doi: 10.1128/jb.170.6.2860-2865.1988.
Abstract
S1 nuclease mapping revealed lacZ mRNA molecules whose 5' and 3' ends were internal to the transcription start and consistent with cleavages at pyrimidine-adenosine bonds 20 to 50 nucleotides apart. With the net 5'-to-3' direction known, lacZ mRNA is probably degraded by sequential cleavages of naked mRNA at vulnerable sites exposed by transit of the last translating ribosome.
摘要
S1核酸酶图谱分析显示，lacZ mRNA分子的5'端和3'端位于转录起始位点内部，并且与相距20至50个核苷酸的嘧啶 - 腺苷键处的切割一致。已知mRNA的净5'到3'方向，lacZ mRNA可能是通过最后一个正在翻译的核糖体通过后暴露的易受攻击位点处的裸露mRNA的顺序切割而降解的。
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