一种用于检测牛冠状病毒的免疫金电子显微镜技术的评估
Evaluation of an immunogold electron microscopy technique for detecting bovine coronavirus.
作者信息
el-Ghorr A A, Snodgrass D R, Scott F M
机构信息
Moredun Research Institute, Edinburgh, Scotland, U.K.
出版信息
J Virol Methods. 1988 Mar-Apr;19(3-4):215-23. doi: 10.1016/0166-0934(88)90016-x.
A solid phase colloidal gold immunoelectron microscopy (IGEM) technique for detecting bovine coronavirus (BCV) was developed and shown to be specific. This test was compared with three other diagnostic tests using fifteen faecal samples. Bovine coronavirus was detected in 2 samples by direct electron microscopy (DEM), in 3 samples by immunosorbent electron microscopy, in 5 samples by haemadsorption-elution-haemagglutination and in 6 samples by IGEM. Ninety four faecal samples were tested by DEM and IGEM. Of 26 samples found to contain BCV by IGEM only 14 were positive by DEM. The IGEM technique is simple, efficient and less susceptible than others to non-specific reactions.
一种用于检测牛冠状病毒（BCV）的固相胶体金免疫电子显微镜（IGEM）技术被开发出来，并证明具有特异性。使用15份粪便样本将该检测方法与其他三种诊断检测方法进行了比较。通过直接电子显微镜（DEM）在2份样本中检测到牛冠状病毒，通过免疫吸附电子显微镜在3份样本中检测到，通过血吸附-洗脱-血凝试验在5份样本中检测到，通过IGEM在6份样本中检测到。通过DEM和IGEM对94份粪便样本进行了检测。在通过IGEM发现含有BCV的26份样本中，只有14份通过DEM呈阳性。IGEM技术简单、高效，且比其他技术更不易发生非特异性反应。
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