Crouch C F, Acres S D
Can J Comp Med. 1984 Jul;48(3):340-2.
The prevalence of rotavirus and coronavirus shedding by adult cows was investigated using capture enzyme-linked immunosorbent assays. Fecal samples from 121 cows in a single herd were tested for the presence of rotavirus and coronavirus, either free or complexed with immunoglobulin. Free rotavirus was not detected in any samples while rotavirus-immunoglobulin complexes were detected in 53 of 121 (44%) samples tested. In contrast, free coronavirus was detected in six (5%) samples and coronavirus-immunoglobulin complexes were detected in 85 (70%) of the samples tested. Thus it appears that subclinical infection of cows by either of these viruses is common, possibly providing a source for infection of the neonate. These assays may therefore provide important information regarding the epidemiology of enteric virus infections and suggest means of improving management to prevent epidemics of neonatal diarrhea.
采用捕获酶联免疫吸附测定法对成年母牛轮状病毒和冠状病毒的排毒率进行了调查。对单个牛群中121头母牛的粪便样本进行检测，以确定是否存在游离或与免疫球蛋白结合的轮状病毒和冠状病毒。在任何样本中均未检测到游离轮状病毒，而在121份检测样本中的53份（44%）中检测到轮状病毒-免疫球蛋白复合物。相比之下，在6份（5%）样本中检测到游离冠状病毒，在85份（70%）检测样本中检测到冠状病毒-免疫球蛋白复合物。因此，这两种病毒对母牛的亚临床感染似乎很常见，可能是新生犊牛感染的一个来源。因此，这些检测方法可能为肠道病毒感染的流行病学提供重要信息，并提示改善管理以预防新生犊牛腹泻流行的方法。
