锌生物玻璃调控人牙髓干细胞的矿化。
Zinc bioglasses regulate mineralization in human dental pulp stem cells.
机构信息
Institute of Dentistry, Barts & The London School of Medicine and Dentistry, Queen Mary University of London, UK; Institute of Dentistry, Dental Physical Sciences Unit, Barts & The London School of Medicine and Dentistry, Queen Mary University of London, UK.
Institute of Dentistry, Dental Physical Sciences Unit, Barts & The London School of Medicine and Dentistry, Queen Mary University of London, UK.
出版信息
Dent Mater. 2017 May;33(5):543-552. doi: 10.1016/j.dental.2017.03.011. Epub 2017 Mar 31.
OBJECTIVE
A promising strategy in regenerative endodontics is the combination of human dental pulp stem cells (hDPSCs) with an appropriate biomaterial substrate. The effects of zinc and zinc containing bioactive glasses (ZnBGs) on hDPSCs have been characterized in this study.
METHODS
ZnBGs were designed and produced. Then the odontogenic differentiation and mineralization potential of hDPSCs upon ZnBGs treatment were investigated.
RESULTS
Free Zn ions (0-5ppm) enhanced proliferation and alkaline phosphatase (ALP) activity of hDPSCs. Further, ZnBGs conditioned medium (ZnBG-CM) increased the production and secretion of odontogenic markers: dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP-1). In addition, we identified that mRNA expression of the osteogenic markers RUNX2, OCN, BSP, BMP-2, MEPE and ON was increased following treatment with ZnBG-CM. Long term treatment with ZnBG-CM increases the formation rate of mineralized nodules (similar to hydroxyapatite, Ca:P=1.6), as confirmed by scanning electron microscopy combined with energy dispersive X-ray spectroscopy (SEM-EDX). Lastly, the administration of ZnBG-CM induces VEGF expression.
SIGNIFICANCE
These findings implicate that ZnBG would be beneficial in regenerative endodontics and could influence the way present Zn containing clinical products are used.
目的
再生牙本质内科学的一个有前途的策略是将人牙髓干细胞（hDPSCs）与合适的生物材料基质相结合。本研究旨在研究锌和含锌的生物活性玻璃（ZnBGs）对 hDPSCs 的影响。
方法
设计并制备了 ZnBGs。然后研究了 hDPSCs 在 ZnBGs 处理下的牙向分化和矿化潜能。
结果
游离锌离子（0-5ppm）可增强 hDPSCs 的增殖和碱性磷酸酶（ALP）活性。此外，ZnBG 条件培养基（ZnBG-CM）增加了牙向标志物的产生和分泌：牙本质涎磷蛋白（DSPP）、牙本质基质蛋白 1（DMP-1）。此外，我们发现，用 ZnBG-CM 处理后，成骨标志物 RUNX2、OCN、BSP、BMP-2、MEPE 和 ON 的 mRNA 表达增加。ZnBG-CM 的长期处理增加了矿化结节的形成率（类似于羟基磷灰石，Ca：P=1.6），这通过扫描电子显微镜结合能谱（SEM-EDX）得到证实。最后，ZnBG-CM 的给药诱导了 VEGF 的表达。
意义
这些发现表明 ZnBG 有益于再生牙本质内科学，并可能影响目前含锌临床产品的使用方式。
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