Wang Ye, Deng Huimin, Li Zhengchao, Tan Yafang, Han Yanping, Wang Xiaoyi, Du Zongmin, Liu Yangyang, Yang Ruifu, Bai Yang, Bi Yujing, Zhi Fachao
Institute of Genetic Engineering, Jinan University,Guangzhou, China; Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Institute of Gastroenterology, Nanfang Hospital, Southern Medical University,Guangzhou, China.
Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Institute of Gastroenterology, Nanfang Hospital, Southern Medical University,Guangzhou, China; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology,Beijing, China.
Front Microbiol. 2017 Mar 17;8:435. doi: 10.3389/fmicb.2017.00435. eCollection 2017.
Commensal non-toxigenic confers powerful health benefits to the host, and has recently been identified as a promising probiotic candidate. We previously isolated strain ZY-312 and identified it as a novel strain based on 16S rRNA sequencing and morphological analyses. We also determined that ZY-312 displayed desirable probiotic properties, including tolerance to simulated digestive fluid, adherence, and safety. In this study, we aim to investigate whether ZY-312 meets the safety criteria required for probiotic bacteria through comprehensive and systematic evaluation. Consequently, the fatty acid profile, metabolite production, and biochemical activity of strain ZY-312 were found to closely resemble descriptions of in Bergey's manual. Taxonomic identification of strain ZY-312 based on whole genome sequencing indicated that ZY-312 and ATCC 25285 showed 99.99% similarity. The 33 putative virulence-associated factors identified in ZY-312 mainly encoded structural proteins and proteins with physiological activity, while the lack of indicated that ZY-312 was non-toxigenic. safety was proven in both normal and immune-deficient mice. The 11 identified antibiotic resistance genes were located on the chromosome rather than on a plasmid, ruling out the risk of plasmid-mediated transfer of antibiotic resistance. , ZY-312 showed resistance to cefepime, kanamycin, and streptomycin. Finally, and notably, ZY-312 exhibited high genetic stability after 100 passages . This study supplements the foundation work on the safety evaluation of ZY-312, and contributes to the development of the first probiotic representative from the dominant Bacteroidetes phylum.
共生非产毒菌对宿主具有强大的健康益处，最近已被确定为一种有前景的益生菌候选菌株。我们之前分离出了ZY - 312菌株，并通过16S rRNA测序和形态学分析将其鉴定为新菌株。我们还确定ZY - 312具有理想的益生菌特性，包括对模拟消化液的耐受性、黏附性和安全性。在本研究中，我们旨在通过全面系统的评估来调查ZY - 312是否符合益生菌所需的安全标准。因此，发现ZY - 312菌株的脂肪酸谱、代谢产物产生和生化活性与《伯杰氏手册》中的描述非常相似。基于全基因组测序对ZY - 312菌株进行的分类鉴定表明，ZY - 312与ATCC 25285的相似度为99.99%。在ZY - 312中鉴定出的33个假定的毒力相关因子主要编码结构蛋白和具有生理活性的蛋白，而缺乏[相关内容未给出]表明ZY - 312是非产毒的。在正常和免疫缺陷小鼠中均证明了其安全性。鉴定出的11个抗生素抗性基因位于染色体上而非质粒上，排除了质粒介导的抗生素抗性转移风险。此外，ZY - 312对头孢吡肟、卡那霉素和链霉素表现出抗性。最后，值得注意的是，ZY - 312在传代100次后表现出高遗传稳定性。本研究补充了ZY - 312安全性评估的基础工作，并有助于开发来自优势拟杆菌门的首个益生菌代表菌株。
