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鼻腔吸附作为一种微创采样方法：原发性呼吸道合胞病毒细支气管炎中的黏膜病毒载量与炎症

Nasosorption as a Minimally Invasive Sampling Procedure: Mucosal Viral Load and Inflammation in Primary RSV Bronchiolitis.

作者信息

Thwaites Ryan S, Ito Kazuhiro, Chingono Jasmine M S, Coates Matthew, Jarvis Hannah C, Tunstall Tanushree, Anderson-Dring Lauren, Cass Lindsey, Rapeport Garth, Openshaw Peter J, Nadel Simon, Hansel Trevor T

机构信息

National Heart and Lung Institute, Faculty of Medicine, Imperial College London.

Pulmocide Ltd, and.

出版信息

J Infect Dis. 2017 Apr 15;215(8):1240-1244. doi: 10.1093/infdis/jix150.

DOI:10.1093/infdis/jix150
PMID:28368490
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5441107/
Abstract

BACKGROUND

Existing respiratory mucosal sampling methods are flawed, particularly in a pediatric bronchiolitis setting.

METHODS

Twenty-four infants with bronchiolitis were recruited: 12 were respiratory syncytial virus (RSV)-positive, 12 were RSV-negative. Infants were sampled by nasosorption and nasopharyngeal aspiration (NPA).

RESULTS

Nasosorption was well tolerated and identified all RSV+ samples. RSV load measured by nasosorption (but not NPA) correlated with length of hospital stay (P = .04) and requirement for mechanical ventilation (P = .03). Nasosorption (but not NPA) levels of interferon γ, interleukin 1β, CCL5/RANTES, and interleukin 10 (IL-10) were elevated in RSV+ bronchiolitis (all P < .05), furthermore CCL5 and IL-10 correlated with RSV load (P < .05).

CONCLUSIONS

Nasosorption allowed measurement of RSV load and the mucosal inflammatory response in infants.

摘要

背景

现有的呼吸道黏膜采样方法存在缺陷，尤其是在小儿细支气管炎的情况下。

方法

招募了24名患有细支气管炎的婴儿：12名呼吸道合胞病毒（RSV）呈阳性，12名RSV呈阴性。通过鼻腔吸附和鼻咽抽吸（NPA）对婴儿进行采样。

结果

鼻腔吸附耐受性良好，且能识别所有RSV阳性样本。通过鼻腔吸附（而非NPA）测得的RSV载量与住院时间（P = 0.04）和机械通气需求（P = 0.03）相关。在RSV阳性的细支气管炎中，鼻腔吸附（而非NPA）检测到的干扰素γ、白细胞介素1β、CCL5/趋化因子调节激活正常T细胞表达和分泌因子（RANTES）以及白细胞介素10（IL-10）水平均升高（所有P < 0.05），此外，CCL5和IL-10与RSV载量相关（P < 0.05）。

结论

鼻腔吸附能够测量婴儿的RSV载量和黏膜炎症反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9860/5441107/a0b6496e9c72/jix15002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9860/5441107/a4531d78971a/jix15001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9860/5441107/a0b6496e9c72/jix15002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9860/5441107/a4531d78971a/jix15001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9860/5441107/a0b6496e9c72/jix15002.jpg

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3
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5
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